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PMID: 1495972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Yeast glycoprotein biosynthesis: MNT1 encodes an alpha-1,2-mannosyltransferase involved in O-glycosylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6846-50

Häusler A, Ballou L, Ballou CE, Robbins PW

Abstract

The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity with alpha-methylmannoside as acceptor. Null mutants of MNT1 are viable, have no apparent growth defect, and are blocked in the elongation of protein O-linked mannobiose. Structural analysis of the N-linked outer chain isolated from an mnn1 mnn10 mnt1 strain revealed no alteration in carbohydrate structure compared to the parental mnn1 mnn10 strain. The MNT1 gene is identical to KRE2, and mutations in the gene render cells resistant to the killer toxin K1 of S. cerevisiae, which suggests a role for O-mannosylated proteins in the resistance mechanism. In addition, MNT1 is part of a multigene family whose members are presumed to be yeast Golgi mannosyltransferases.

Related Genes
KRE2 MNT1
MeSH Terms
Carbohydrate Sequence Chromatography, Paper Genes, Fungal Glycoproteins/biosynthesis Glycosylation Mannosidases/metabolism Mannosyltransferases/genetics,metabolism Molecular Sequence Data Multigene Family Oligosaccharides/biosynthesis,isolation & purification Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Glycoproteins Oligosaccharides Saccharomyces cerevisiae Proteins KRE2 protein, S cerevisiae Mannosyltransferases Mannosidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Häusler A
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Ballou L
Ballou C E
Robbins P W
References (31)
31 references, click to expand
  1. Dolichol phosphate mannose synthase is required in vivo for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and N glycosylation of protein in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Nov;10(11):5796-805 PMID: 2146492
  2. Separation and characterization of two alpha 1,2-mannosyltransferase activities from Saccharomyces cerevisiae.
    J Biol Chem. 1991 May 5;266(13):8255-61 PMID: 2022642
  3. Separation of yeast asparagine-linked oligosaccharides by high-performance anion-exchange chromatography.
    Carbohydr Res. 1990 Aug 1;203(1):1-11 PMID: 2224897
  4. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  5. Protein glycosylation defects in the Saccharomyces cerevisiae mnn7 mutant class. Support for the stop signal proposed for regulation of outer chain elongation.
    J Biol Chem. 1989 Jul 15;264(20):11857-64 PMID: 2663843
  6. Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation.
    J Biol Chem. 1989 Oct 25;264(30):17615-8 PMID: 2681181
  7. Glycobiology.
    Annu Rev Biochem. 1988;57:785-838 PMID: 3052290
  8. Protein glycosylation in yeast.
    Annu Rev Biochem. 1987;56:915-44 PMID: 3304149
  9. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  10. O-glycosylation in Saccharomyces cerevisiae is initiated at the endoplasmic reticulum.
    FEBS Lett. 1983 Jul 25;158(2):335-8 PMID: 6347716
  11. The role of S. cerevisiae cell division cycle genes in nuclear fusion.
    Genetics. 1982 Feb;100(2):175-84 PMID: 7049831
  12. Synthesis of an O-glycosylated cell surface protein induced in yeast by alpha factor.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6263-6 PMID: 16593749
  13. Yeast manno-protein biosynthesis: solubilization and selective assay of four mannosyltransferases.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3912-6 PMID: 1105568
  14. Carbohydrate ligands of the LEC cell adhesion molecules.
    Cell. 1990 Nov 30;63(5):861-3 PMID: 1701693
  15. Partial purification of a mannosyltransferase involved in the O-mannosylation of glycoproteins from Saccharomyces cerevisiae.
    Glycobiology. 1991 Sep;1(4):367-73 PMID: 1820198
  16. Analysis of glycoproteins from Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:682-97 PMID: 2005816
  17. The Saccharomyces cerevisiae RPB4 gene is tightly linked to the TIF2 gene.
    Nucleic Acids Res. 1991 May 25;19(10):2781 PMID: 2041753
  18. Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects.
    Methods Enzymol. 1990;185:440-70 PMID: 2199792
  19. Glycoprotein biosynthesis in Saccharomyces cerevisiae. Characterization of alpha-1,6-mannosyltransferase which initiates outer chain formation.
    J Biol Chem. 1989 Feb 5;264(4):1946-50 PMID: 2644248
  20. A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure.
    J Biol Chem. 1989 Jul 15;264(20):11849-56 PMID: 2663842
  21. Purification and characterization of the inducible a agglutinin of Saccharomyces cerevisiae.
    EMBO J. 1988 May;7(5):1483-8 PMID: 3044782
  22. Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein.
    J Biol Chem. 1988 Nov 25;263(33):17499-507 PMID: 3053713
  23. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  24. Saccharomyces cerevisiae mannoprotein mutants. Isolation of the mnn5 mutant and comparison with the mnn3 strain.
    J Biol Chem. 1980 Aug 25;255(16):7700-7 PMID: 6995454
  25. Detection of sugars on paper chromatograms.
    Nature. 1950 Sep 9;166(4219):444-5 PMID: 14775715
  26. Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins.
    Genetics. 1992 Feb;130(2):273-83 PMID: 1541391
  27. Glycosylation in Saccharomyces cerevisiae: cloning and characterization of an alpha-1,2-mannosyltransferase structural gene.
    Glycobiology. 1992 Feb;2(1):77-84 PMID: 1550992
  28. K1 killer toxin, a pore-forming protein from yeast.
    Mol Microbiol. 1991 Oct;5(10):2339-43 PMID: 1724277
  29. Chitinase is required for cell separation during growth of Saccharomyces cerevisiae.
    J Biol Chem. 1991 Oct 15;266(29):19758-67 PMID: 1918080
  30. Protein O-glycosylation in Saccharomyces cerevisiae. Purification and characterization of the dolichyl-phosphate-D-mannose-protein O-D-mannosyltransferase.
    Eur J Biochem. 1991 Feb 26;196(1):185-90 PMID: 2001697
  31. Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3368-72 PMID: 2185468
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6846
Published
1992-08-01
Pages
6846-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49601
Subset
IM
Grants
NCI NIH HHS · CA14051 · United States
NIGMS NIH HHS · GM45188 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.15.6846 PMC Full Text View journal details
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