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PMID: 1495984 Published · ppublish English Journal Article

Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6943-7

McKnight RA, Shamay A, Sankaran L, Wall RJ, Hennighausen L

Abstract

Matrix-attachment regions (MARs) may function as domain boundaries and partition chromosomes into independently regulated units. We have tested whether MAR sequences from the chicken lysozyme locus, the so-called A-elements, can confer position-independent regulation to a whey acidic protein (WAP) transgene in mammary tissue of mice. In the absence of MARs, expression of WAP transgenes was observed in 50% of the lines, and regulation during pregnancy, during lactation, and upon hormonal induction did not mimic that of the endogenous WAP gene and varied with the integration site. In contrast, all 11 lines in which WAP transgenes were juxtaposed to MAR elements showed expression. Accurate position-independent hormonal and developmental regulation was seen in four out of the five lines analyzed. These results indicate that MARs can establish independent genetic domains in transgenic mice.

Related Genes
WAP
MeSH Terms
Animals Binding Sites Caseins/genetics Chickens Female Lactation Mammary Glands, Animal/growth & development,physiology Mice Mice, Transgenic Milk Proteins/genetics,metabolism Muramidase/genetics Oligonucleotide Probes Organ Culture Techniques Polymerase Chain Reaction Pregnancy RNA, Messenger/genetics,metabolism Restriction Mapping
Chemicals
Caseins Milk Proteins Oligonucleotide Probes RNA, Messenger whey acidic proteins Muramidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McKnight R A
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20982.
Shamay A
Sankaran L
Wall R J
Hennighausen L
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6943
Published
1992-08-01
Pages
6943-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49621
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.15.6943 PMC Full Text View journal details
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