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PMID: 1500194 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Iron acquisition and hemolysin production by Campylobacter jejuni.

Infection and immunity ·Vol. 60 ·No. 9 ·1992-09-00 ·Pages 3872-7

Pickett CL, Auffenberg T, Pesci EC, Sheen VL, Jusuf SS

Abstract

Campylobacter jejuni strains were tested for their ability to acquire iron from various iron sources present in humans. Hemin, hemoglobin, hemin-hemopexin, and hemoglobin-haptoglobin stimulated the growth of C. jejuni strains in low-iron medium. Transferrin, lactoferrin, and ferritin were unable to provide iron to the strains tested. Derivatives of the naturally transformable C. jejuni strain 81-176 were isolated on the basis of their inability to use hemin as an iron source. These mutants were also unable to use hemoglobin, hemin-hemopexin, or hemoglobin-haptoglobin as iron sources. Some mutants lacked a 71,000-Da iron-regulated outer membrane protein, while others appeared to retain all of their outer membrane proteins. Growth curves and a recombination experiment that exploited natural transformation were used to further characterize the mutants. A hemolytic activity was shown to be produced by several C. jejuni strains, but it did not appear to be iron regulated.

MeSH Terms
Campylobacter jejuni/growth & development,metabolism Hemin/pharmacology Hemolysin Proteins/biosynthesis Iron/metabolism Mutation
Chemicals
Hemolysin Proteins Hemin Iron
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pickett C L
Department of Microbiology and Immunology, Chandler Medical Center, University of Kentucky, Lexington 40536-0084.
Auffenberg T
Pesci E C
Sheen V L
Jusuf S S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.9.3872-3877.1992
Published
1992-09-00
Pages
3872-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257401
Subset
IM
Grants
NIAID NIH HHS · AI-27908 · United States
External Links
PubMed source DOI: 10.1128/iai.60.9.3872-3877.1992 PMC Full Text View journal details
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