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PMID: 1500851 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Association of the tyrosine kinase LCK with phospholipase C-gamma 1 after stimulation of the T cell antigen receptor.

The Journal of experimental medicine ·Vol. 176 ·No. 2 ·1992-08-01 ·Pages 373-9

Weber JR, Bell GM, Han MY, Pawson T, Imboden JB

Abstract

Stimulation of the T cell antigen receptor (TCR) activates a protein tyrosine kinase and leads to the tyrosine phosphorylation of phosphoinositide-specific phospholipase C-gamma 1 (PLC gamma 1). The molecular interactions involved in this phosphorylation are not known. After stimulation of the TCR on Jurkat T cells, tyrosine-phosphorylated proteins of 36, 38, 58, and 63 kD coprecipitate with PLC gamma 1. An identical pattern of proteins precipitate with TrpE fusion proteins that contain the Src homology (SH) 2 domains of PLC gamma 1, indicating that these regions of PLC gamma 1 are responsible for binding. TCR stimulation leads to an association between the SH2 domains of PLC gamma 1 and a protein tyrosine kinase, which, by peptide mapping, is identical to p56lck. These studies establish that p56lck associates with PLC gamma 1 as a result of TCR stimulation of Jurkat cells, suggesting that p56lck plays a central role in coupling the TCR to the activation of PLC gamma 1.

MeSH Terms
Cell Line Humans Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Peptide Mapping Phosphorylation Protein-Tyrosine Kinases/metabolism Receptors, Antigen, T-Cell/metabolism T-Lymphocytes/enzymology,metabolism Type C Phospholipases/metabolism Tyrosine/metabolism
Chemicals
Receptors, Antigen, T-Cell Tyrosine Protein-Tyrosine Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Type C Phospholipases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Weber J R
Department of Medicine, University of California, San Francisco.
Bell G M
Han M Y
Pawson T
Imboden J B
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.176.2.373
Published
1992-08-01
Pages
373-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2119313
Subset
IM
Grants
NIDDK NIH HHS · DK-07007 · United States
NIAID NIH HHS · R01 AI-26644 · United States
External Links
PubMed source DOI: 10.1084/jem.176.2.373 PMC Full Text View journal details
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