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PMID: 15037762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Coupling morphogenesis to mitotic entry.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 12 ·2004-03-23 ·Pages 4124-9

Sakchaisri K, Asano S, Yu LR, Shulewitz MJ, Park CJ, Park JE, Cho YW, Veenstra TD, Thorner J, Lee KS

Abstract

In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G(2)/M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.

MeSH Terms
Cell Cycle Proteins/metabolism Mitosis/physiology Morphogenesis/physiology Phosphorylation Protein Kinases/metabolism Protein Serine-Threonine Kinases/metabolism Protein-Tyrosine Kinases/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Cell Cycle Proteins Saccharomyces cerevisiae Proteins Protein Kinases SWE1 protein, S cerevisiae Protein-Tyrosine Kinases CLA4 protein, S cerevisiae Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Sakchaisri Krisada
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Bethesda, MD 20892, USA.
Asano Satoshi
Yu Li-Rong
Shulewitz Mark J
Park Chong J
Park Jung-Eun
Cho Young-Wook
Veenstra Timothy D
Thorner Jeremy
Lee Kyung S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0400641101
Published
2004-03-23
Epub
2004-00-22
Pages
4124-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384705
Subset
IM
Grants
NIGMS NIH HHS · R01 GM021841 · United States
NIGMS NIH HHS · GM 21841 · United States
External Links
PubMed source DOI: 10.1073/pnas.0400641101 PMC Full Text View journal details
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