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PMID: 8253069 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins.

The EMBO journal ·Vol. 12 ·No. 9 ·1993-09-00 ·Pages 3417-26

Booher RN, Deshaies RJ, Kirschner MW

Abstract

Wee1 is a protein kinase that negatively regulates p34cdc2 kinase activity. We have identified a Saccharomyces cerevisiae wee1 homolog encoded by the SWE1 gene. SWE1 overexpression arrests cells in G2 with short spindles whereas deletion of SWE1 did not alter the cell cycle but did eliminate the G2 delay observed in mih1- mutants. Swe1 immunoprecipitates were capable of tyrosine phosphorylating and inactivating p34CDC28 complexed with Clb2, a G2-type cyclin, but not p34CDC28 complexed with Cln2, a G1-type cyclin, consistent with the inability of Swe1 overexpression to inhibit the G1/S transition. These results suggest that specific cyclin subunits target p34CDC28 for distinct regulatory controls which may be important for ensuring proper p34CDC28 function during the cell cycle.

Related Genes
SWE1
MeSH Terms
Amino Acid Sequence Base Sequence CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle Proteins Cyclins/metabolism G1 Phase G2 Phase Genes, Fungal Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins Oligodeoxyribonucleotides Protein Kinases/metabolism Protein-Tyrosine Kinases Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,metabolism Schizosaccharomyces/genetics,growth & development Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid
Chemicals
Cell Cycle Proteins Cyclins Nuclear Proteins Oligodeoxyribonucleotides Schizosaccharomyces pombe Proteins Protein Kinases wee1 protein, S pombe Protein-Tyrosine Kinases CDC28 Protein Kinase, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Booher R N
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Deshaies R J
Kirschner M W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-09-00
Pages
3417-26
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413617
Subset
IM
Grants
NCI NIH HHS · 1F32CA08789 · United States
Databases
GENBANK
X73966
External Links
PubMed source PMC Full Text View journal details
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