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PMID: 15084657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Endoplasmic reticulum export site formation and function in dendrites.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 24 ·No. 15 ·2004-04-14 ·Pages 3770-6

Aridor M, Guzik AK, Bielli A, Fish KN

Abstract

The elongated and polarized characteristics of neurons render targeting of receptors to the plasma membrane of distal axonal projections and dendritic branches a major sorting task. Although the majority of biosynthetic cargo synthesis, transport, and sorting are believed to occur in the soma, local membrane protein translation and sorting has been reported recently to take place in dendrites and axons. We investigated where endoplasmic reticulum (ER) export occurs in dendrites using an in vitro permeabilized neuron system that enables us to specifically control the assembly of ER export sites. We show that ER export sites are assembled regularly throughout the entire dendritic tree by the regulated sequential recruitment of Sar1 and COPII (coat protein complex II). Moreover, activation of metabotropic glutamate receptors leads to the recruitment of the NMDA receptor subunit NR1 to remodeled ER export sites. We propose that regulation of receptor assembly and export from the ER in dendrites plays an important role in modulating receptor surface expression and neuronal function.

MeSH Terms
Animals COP-Coated Vesicles/metabolism Cell Membrane Permeability Cells, Cultured Dendrites/drug effects,physiology,ultrastructure Endoplasmic Reticulum/drug effects,metabolism Excitatory Amino Acid Agents/pharmacology Glycine/analogs & derivatives,pharmacology Macromolecular Substances Membrane Proteins/metabolism Monomeric GTP-Binding Proteins/genetics,metabolism Nuclear Pore Complex Proteins Protein Transport/physiology Rats Rats, Sprague-Dawley Receptors, Metabotropic Glutamate/agonists,metabolism Receptors, N-Methyl-D-Aspartate/metabolism Resorcinols/pharmacology Saccharomyces cerevisiae Proteins
Chemicals
Excitatory Amino Acid Agents Macromolecular Substances Membrane Proteins NR1 NMDA receptor Nuclear Pore Complex Proteins Receptors, Metabotropic Glutamate Receptors, N-Methyl-D-Aspartate Resorcinols SEC13 protein, S cerevisiae Saccharomyces cerevisiae Proteins 3,5-dihydroxyphenylglycine SAR1 protein, rat Monomeric GTP-Binding Proteins Glycine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aridor Meir
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
Guzik Amy K
Bielli Anna
Fish Kenneth N
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.4775-03.2004
Published
2004-04-14
Pages
3770-6
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6729346
Subset
IM
Grants
NIMH NIH HHS · MH064372 · United States
NIDDK NIH HHS · R01 DK062318 · United States
NIMH NIH HHS · K01 MH064372-02 · United States
NIMH NIH HHS · K01 MH064372-01 · United States
NIMH NIH HHS · K01 MH064372-03 · United States
NIMH NIH HHS · K01 MH064372-05 · United States
NIMH NIH HHS · K01 MH064372 · United States
NIMH NIH HHS · K01 MH064372-04 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.4775-03.2004 PMC Full Text View journal details
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