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PMID: 1510445 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of peptidoglycan precursors in vancomycin-resistant enterococci.

Antimicrobial agents and chemotherapy ·Vol. 36 ·No. 7 ·1992-07-00 ·Pages 1487-90

Billot-Klein D, Gutmann L, Collatz E, van Heijenoort J

Abstract

Analysis by high-pressure liquid chromatography of the cytoplasmic peptidoglycan precursors of a high- and a low-level vancomycin-resistant Enterococcus spp. was performed before and after induction of resistance. This analysis showed a decrease of the D-Ala-D-Ala and UDP-MurNac-pentapeptide pools, an increase of the UDP-MurNac-tripeptide pool, and the appearance of new UDP-MurNac-containing material. These results lead us to suggest that the vancomycin-induced carboxypeptidase activity cleaves the D-Ala-D-Ala (L. Gutmann, D. Billot-Klein, S. Al-Obeid, I. Klare, S. Francoul, E. Collatz, and J. van Heijenoort, Antimicrob. Agents Chemother. 36:77-80, 1992), which in turn would prevent formation of the normal UDP-MurNac-pentapeptide and thereby of the vancomycin target. The novel UDP-MurNac-containing material is thought to correspond to peptidoglycan precursors which might be synthesized by an alternate pathway (T. D. H. Bugg, G. D. Wright, S. Dutka-Malen, M. Arthur, P. Courvalin, and C. T. Walsh, Biochemistry 30:10408-10415, 1991) and which would be unable to bind vancomycin in glycopeptide-resistant enterococci.

MeSH Terms
Cells, Cultured Chromatography, High Pressure Liquid Dipeptides/analysis Drug Resistance, Microbial Enterococcus/drug effects,metabolism Peptidoglycan/biosynthesis Uridine Diphosphate N-Acetylmuramic Acid/analogs & derivatives,analysis Vancomycin/pharmacology
Chemicals
Dipeptides Peptidoglycan Uridine Diphosphate N-Acetylmuramic Acid UDP-N-acetylmuramic acid pentapeptide alanylalanine Vancomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Billot-Klein D
Laboratoire de Microbiologie Médicale, Université Paris VI, France.
Gutmann L
Collatz E
van Heijenoort J
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18 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.36.7.1487
Published
1992-07-00
Pages
1487-90
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC191609
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.36.7.1487 PMC Full Text View journal details
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