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PMID: 15121898 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

The composition of Staufen-containing RNA granules from human cells indicates their role in the regulated transport and translation of messenger RNAs.

Nucleic acids research ·Vol. 32 ·No. 8 ·2004-00-00 ·Pages 2411-20

Villacé P, Marión RM, Ortín J

Abstract

hStaufen is the human homolog of dmStaufen, a double-stranded (ds)RNA-binding protein involved in early development of the fly. hStaufen-containing complexes were purified by affinity chromatography from human cells transfected with a TAP-tagged hStaufen gene. These complexes showed a size >10 MDa. Untagged complexes with similar size were identified from differentiated human neuroblasts. The identity of proteins present in purified hStaufen complexes was determined by mass spectrometry and the presence of these proteins and other functionally related ones was verified by western blot. Ribosomes and proteins involved in the control of protein synthesis (PABP1 and FMRP) were present in purified hStaufen complexes, as well as elements of the cytoskeleton (tubulins, tau, actin and internexin), cytoskeleton control proteins (IQGAP1, cdc42 and rac1) and motor proteins (dynein, kinesin and myosin). In addition, proteins normally found in the nucleus, like nucleolin and RNA helicase A, were also found associated with cytosolic hStaufen complexes. The co-localization of these components with hStaufen granules in the dendrites of differentiated neuroblasts, determined by confocal immunofluorescence, validated their association in living cells. These results support the notion that the hStaufen-containing granules are structures essential in the localization and regulated translation of human mRNAs in vivo.

MeSH Terms
ATP-Binding Cassette Transporters Biological Transport Cell Line Cytoplasmic Granules/chemistry Cytoskeletal Proteins/analysis Histocompatibility Antigens Class I/genetics Humans Macromolecular Substances Microtubule-Associated Proteins/analysis Neurons/chemistry,metabolism Nuclear Proteins/analysis Protein Biosynthesis RNA/analysis RNA, Messenger/metabolism RNA-Binding Proteins/analysis,genetics,physiology Recombinant Fusion Proteins/isolation & purification,metabolism Ribosomes/metabolism
Chemicals
ATP-Binding Cassette Transporters Cytoskeletal Proteins Histocompatibility Antigens Class I Macromolecular Substances Microtubule-Associated Proteins Nuclear Proteins RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins STAU1 protein, human staufen protein, mammalian transporter associated with antigen processing (TAP) RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Villacé Patricia
Centro Nacional de Biotecnología, Campus de Cantoblanco, 28049 Madrid, Spain.
Marión Rosa M
Ortín Juan
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh552
Published
2004-00-00
Epub
2004-00-30
Pages
2411-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC419443
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkh552 PMC Full Text View journal details
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