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PMID: 15155878 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis Rab GTPase RabA4b localizes to the tips of growing root hair cells.

The Plant cell ·Vol. 16 ·No. 6 ·2004-06-00 ·Pages 1589-603

Preuss ML, Serna J, Falbel TG, Bednarek SY, Nielsen E

Abstract

Spatial and temporal control of cell wall deposition plays a unique and critical role during growth and development in plants. To characterize membrane trafficking pathways involved in these processes, we have examined the function of a plant Rab GTPase, RabA4b, during polarized expansion in developing root hair cells. Whereas a small fraction of RabA4b cofractionated with Golgi membrane marker proteins, the majority of this protein labeled a unique membrane compartment that did not cofractionate with the previously characterized trans-Golgi network syntaxin proteins SYP41 and SYP51. An enhanced yellow fluorescent protein (EYFP)-RabA4b fusion protein specifically localizes to the tips of growing root hair cells in Arabidopsis thaliana. Tip-localized EYFP-RabA4b disappears in mature root hair cells that have stopped expanding, and polar localization of the EYFP-RabA4b is disrupted by latrunculin B treatment. Loss of tip localization of EYFP-RabA4b was correlated with inhibition of expansion; upon washout of the inhibitor, root hair expansion recovered only after tip localization of the EYFP-RabA4b compartments was reestablished. Furthermore, in mutants with defective root hair morphology, EYFP-RabA4b was improperly localized or was absent from the tips of root hair cells. We propose that RabA4b regulates membrane trafficking through a compartment involved in the polarized secretion of cell wall components in plant cells.

MeSH Terms
Actins/metabolism Arabidopsis/cytology,enzymology,genetics,growth & development Cell Polarity Cytoskeleton/metabolism Gene Expression Profiling Intracellular Membranes/metabolism Microtubules/metabolism Mutation/genetics Plant Roots/cytology,enzymology,growth & development,metabolism Protein Transport rab GTP-Binding Proteins/metabolism
Chemicals
Actins rab GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Preuss Mary L
Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.
Serna Jannie
Falbel Tanya G
Bednarek Sebastian Y
Nielsen Erik
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.021634
Published
2004-06-00
Epub
2004-00-21
Pages
1589-603
Language
English
Region
England
NLM ID
9208688
PMCID
PMC490048
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.021634 PMC Full Text View journal details
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