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PMID: 15185861 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

In vivo effects of bifidobacteria and lactoferrin on gut endotoxin concentration and mucosal immunity in Balb/c mice.

Digestive diseases and sciences ·Vol. 49 ·No. 4 ·2004-04-00 ·Pages 579-89

Griffiths EA, Duffy LC, Schanbacher FL, Qiao H, Dryja D, Leavens A, Rossman J, Rich G, Dirienzo D, Ogra PL

Abstract

The aim of the present study was to examine the effects of oral supplementation of newborn Balb/c mice with bifidobacteria (B. infantis, B. bifidum) and iron-free apo-lactoferrin (bovine, human) on gut endotoxin concentration and mucosal immunity. Endotoxin concentration was measured in ileocecal filtrates at 7, 14, 21, and 28 days postdelivery by a quantitative limulus amebocyte lysate test. While endotoxin levels in bifidobacteria-fed mice showed a steady rise over time, they were consistently lower than that observed in control animals. Results of lactoferrin supplementation varied depending on the specific time point, but overall by day 28, all treatment groups showed lower intestinal endotoxin concentrations compared to saline fed animals. Neither bifidobacteria nor lactoferrin stimulated an increase in B or T cells, or in cytokine production (IL-6, TNF-alpha, INF-gamma), in Peyer's patches as measured by flow cytometry. Bifidobacteria and lactoferrin were well tolerated as dietary supplements and showed promising potential to reduce gut endotoxin levels.

MeSH Terms
Administration, Oral Analysis of Variance Animals Bifidobacterium Biomarkers Culture Techniques Endotoxins/analysis,metabolism Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Immunity, Mucosal/physiology Interferon-gamma/analysis Interleukin-6/analysis Intestinal Mucosa/immunology,microbiology Intestines/drug effects,immunology Lactoferrin/pharmacology Limulus Test Mice Mice, Inbred BALB C Pregnancy Pregnancy, Animal Probability Reference Values Risk Factors Sensitivity and Specificity Tumor Necrosis Factor-alpha/analysis
Chemicals
Biomarkers Endotoxins Interleukin-6 Tumor Necrosis Factor-alpha Interferon-gamma Lactoferrin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Griffiths Elizabeth A
Department of Pediatrics, The Women and Children's Hospital of Buffalo, Kaleida Health, School of Medicine, State University of New York at Buffalo, 888 Delaware Avenue, Buffalo, New York 14209, USA.
Duffy Linda C
Schanbacher Floyd L
Qiao Haiping
Dryja Diane
Leavens Allen
Rossman Jon
Rich Gary
Dirienzo Douglas
Ogra Pearay L
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Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1023/b:ddas.0000026302.92898.ae
Published
2004-04-00
Pages
579-89
Language
English
Region
United States
NLM ID
7902782
Subset
IM
External Links
PubMed source DOI: 10.1023/b:ddas.0000026302.92898.ae View journal details
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