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PMID: 1518836 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulated expression of the human acetylated low density lipoprotein receptor gene and isolation of promoter sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 17 ·1992-09-01 ·Pages 8102-6

Moulton KS, Wu H, Barnett J, Parthasarathy S, Glass CK

Abstract

The acetylated low density lipoprotein (AcLDL) receptor is expressed on tissue macrophages after their differentiation from monocyte precursors and has been proposed to play a role in the generation of foam cells in atherosclerotic lesions. In the present studies, THP-1 human monocytic leukemia cells were used to investigate mechanisms responsible for expression of the AcLDL receptor gene after treatment with phorbol 12-myristate 13-acetate (TPA). TPA-dependent accumulation of AcLDL receptor mRNA was not detected until after a lag phase of 12 hr and was blocked by concurrent treatment with cycloheximide. In addition, the TPA-dependent induction of AcLDL receptor activity and mRNA levels was inhibited by retinoic acid and dexamethasone treatment. Isolation and sequence analysis of the promoter regions for the human and bovine AcLDL receptor genes indicated high sequence similarity. Binding sites for AP-1 proteins or other known transcription factors were not conserved between the two species, suggesting that novel factors are required for AcLDL receptor expression.

MeSH Terms
Base Sequence Cell Adhesion Molecules Cell Nucleus/metabolism Cells, Cultured Cloning, Molecular DNA/genetics Gene Expression Regulation Genes Humans In Vitro Techniques Macrophages/physiology Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Promoter Regions, Genetic RNA, Messenger/genetics Receptors, LDL/genetics Receptors, Scavenger Sequence Alignment Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic
Chemicals
Cell Adhesion Molecules Oligodeoxyribonucleotides RNA, Messenger Receptors, LDL Receptors, Scavenger DNA Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moulton K S
Department of Medicine, University of California, San Diego, La Jolla 92093-0656.
Wu H
Barnett J
Parthasarathy S
Glass C K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.17.8102
Published
1992-09-01
Pages
8102-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49864
Subset
IM
Databases
GENBANK
M93188, M93189
External Links
PubMed source DOI: 10.1073/pnas.89.17.8102 PMC Full Text View journal details
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