Home LiteratureArticle Details
PMID: 3259318 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A conserved sequence in the T-cell receptor beta-chain promoter region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 10 ·1988-05-00 ·Pages 3551-4

Anderson SJ, Chou HS, Loh DY

Abstract

The antigen-specific receptors of T and B lymphocytes are distinct, though structurally related, molecules. During development, lymphoid cells assemble functional variable (V) region genes for each receptor chain from separate multimember gene families by somatic DNA rearrangements of individual germ-line segments. Transcription may play a role in regulating the tissue and stage specificity of these rearrangements by controlling the accessibility of germ-line loci to the recombinational machinery. Immunoglobulin V-region genes are transcribed from tissue-specific promoters that have been well characterized. We report here the characterization of 14 T-cell receptor beta-chain V-region gene promoters. Sequence analysis indicates that these promoters do not contain the conserved octamer that is located upstream of all immunoglobulin genes. However, a unique decanucleotide sequence, not present in immunoglobulin genes, is conserved in the promoter region of murine and human V beta genes. We identify this sequence as a potential regulatory element, based on its position, conservation, and sequence homology to sites known to bind transcription-activating factors. The possibility that the distinct structures of immunoglobulin and T-cell receptor gene promoters may contribute to the tissue-specific rearrangement and expression of receptor gene families is discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular Genes Genes, Immunoglobulin Immunoglobulin Variable Region/genetics Macromolecular Substances Mice Molecular Sequence Data Promoter Regions, Genetic Receptors, Antigen, T-Cell/genetics T-Lymphocytes/immunology Transcription, Genetic
Chemicals
Immunoglobulin Variable Region Macromolecular Substances Receptors, Antigen, T-Cell
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anderson S J
Howard Hughes Medical Institute, Department of Medicine, Washington University Medical School, St. Louis, MO 63110.
Chou H S
Loh D Y
References (40)
40 references, click to expand
  1. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  2. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
    Nature. 1987 Jul 9-15;328(6126):175-8 PMID: 2885756
  3. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  4. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  5. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  6. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  7. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  8. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  9. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  10. Two regulatory elements for immunoglobulin kappa light chain gene expression.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5 PMID: 6438631
  11. Developmentally controlled and tissue-specific expression of unrearranged VH gene segments.
    Cell. 1985 Feb;40(2):271-81 PMID: 2578321
  12. Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence.
    Cell. 1985 Jun;41(2):479-87 PMID: 3921262
  13. T-cell receptor beta-chain expression: dependence on relatively few variable region genes.
    Science. 1985 Aug 9;229(4713):566-70 PMID: 3875151
  14. The murine T-cell receptor uses a limited repertoire of expressed V beta gene segments.
    Nature. 1985 Aug 8-14;316(6028):517-23 PMID: 2412120
  15. Structure of the human interleukin-2 receptor gene.
    Science. 1985 Nov 8;230(4726):633-9 PMID: 2996141
  16. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  17. Introduced T cell receptor variable region gene segments recombine in pre-B cells: evidence that B and T cells use a common recombinase.
    Cell. 1986 Jan 31;44(2):251-9 PMID: 3484682
  18. Murine T-cell receptor mutants with deletions of beta-chain variable region genes.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):767-71 PMID: 3456168
  19. Regulation of genome rearrangement events during lymphocyte differentiation.
    Immunol Rev. 1986 Feb;89:5-30 PMID: 3081433
  20. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition.
    Annu Rev Immunol. 1986;4:529-91 PMID: 3518750
  21. Tandem kappa immunoglobulin promoters are equally active in the presence of the kappa enhancer: implications for models of enhancer function.
    Cell. 1986 Jul 18;46(2):253-62 PMID: 3087628
  22. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  23. Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6382-6 PMID: 3462701
  24. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  25. Analysis of a human V beta gene subfamily.
    J Exp Med. 1986 Nov 1;164(5):1600-14 PMID: 3772297
  26. A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
    Nature. 1986 Oct 16-22;323(6089):640-3 PMID: 3095662
  27. Cell-type-specific transcription of an immunoglobulin kappa light chain gene in vitro.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8511-5 PMID: 3095838
  28. T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments.
    EMBO J. 1986 Sep;5(9):2149-55 PMID: 3490968
  29. Recombination between immunoglobulin variable region gene segments is enhanced by transcription.
    Nature. 1986 Dec 11-17;324(6097):585-9 PMID: 3491327
  30. Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions.
    Nucleic Acids Res. 1986 Nov 25;14(22):8829-44 PMID: 3786139
  31. Multiple nuclear proteins bind upstream sequences in the promotor region of a T-cell receptor beta-chain variable-region gene: evidence for tissue specificity.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):232-6 PMID: 3025857
  32. Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40.
    Nature. 1987 Jan 22-28;325(6102):368-72 PMID: 3027570
  33. Continuing rearrangement of immunoglobulin and T-cell receptor genes in a Ha-ras-transformed lymphoid progenitor cell line.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1799-803 PMID: 3470759
  34. Tandem linkage and unusual RNA splicing of the T-cell receptor beta-chain variable-region genes.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1992-6 PMID: 3470773
  35. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  36. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  37. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals.
    Cell. 1987 Jun 19;49(6):775-83 PMID: 3495343
  38. Receptor diversity of insulin-specific T cell lines from C57BL (H-2b) mice.
    J Immunol. 1987 Jun 1;138(11):3991-5 PMID: 2953795
  39. Protein kinase C-activating phorbol esters augment expression of T cell receptor genes.
    Eur J Immunol. 1987 Jun;17(6):803-7 PMID: 3496224
  40. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.10.3551
Published
1988-05-00
Pages
3551-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280251
Subset
IM
Grants
NIAID NIH HHS · AI07163 · United States
NIGMS NIH HHS · GM07067 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.10.3551 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2