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PMID: 3456168 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Murine T-cell receptor mutants with deletions of beta-chain variable region genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 3 ·1986-02-00 ·Pages 767-71

Behlke MA, Chou HS, Huppi K, Loh DY

Abstract

Genomic Southern blots of DNA from eight strains of mice were examined for restriction fragment length polymorphisms in their loci encoding the variable region of the T-cell receptor beta chain (V beta), using 16 different V beta-specific probes. Mouse strains BALB/c, C57BL/6, C3H, and PL were identical, while strains SJL, C57BR, C57L, and SWR shared several polymorphisms with respect to the other four strains. In addition, SJL, C57L, C57BR, and SWR DNAs were missing 50% of the hybridizing bands visualized in BALB/c DNA. A cDNA library from concanavalin A-stimulated SJL spleen blasts was constructed and examined for V beta gene usage. Ten genes were found to account for all V beta-containing clones isolated, including three newly identified V beta genes. All 10 of these genes were found to be present in BALB/c mice. We conclude that SJL, C57L, C57BR, and SWR mice represent V beta deletion mutants of the BALB/c genotype.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred Strains Mutation Nucleic Acid Hybridization Polymorphism, Genetic Rats Receptors, Antigen, T-Cell/genetics
Chemicals
Antibodies, Monoclonal Immunoglobulin Variable Region Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Behlke M A
Chou H S
Huppi K
Loh D Y
References (27)
27 references, click to expand
  1. Genetic markers of the antigen-specific T cell receptor locus.
    J Exp Med. 1985 May 1;161(5):1219-24 PMID: 2580936
  2. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  3. Genetic polymorphism at the kappa chain locus in mice: comparisons of restriction enzyme hybridization fragments of variable and constant region genes.
    Immunogenetics. 1985;21(5):445-57 PMID: 2987114
  4. Identical V beta T-cell receptor genes used in alloreactive cytotoxic and antigen plus I-A specific helper T cells.
    Nature. 1985 May 30-Jun 5;315(6018):425-7 PMID: 2582268
  5. V beta gene polymorphism and a major polyclonal T cell receptor idiotype.
    Cell. 1985 Aug;42(1):89-92 PMID: 2410144
  6. T-cell receptor beta-chain expression: dependence on relatively few variable region genes.
    Science. 1985 Aug 9;229(4713):566-70 PMID: 3875151
  7. The murine T-cell receptor uses a limited repertoire of expressed V beta gene segments.
    Nature. 1985 Aug 8-14;316(6028):517-23 PMID: 2412120
  8. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  9. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  10. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  11. Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins.
    Nature. 1984 Mar 8-14;308(5955):153-8 PMID: 6546606
  12. Somatic recombination in a murine T-cell receptor gene.
    Nature. 1984 May 24-30;309(5966):322-6 PMID: 6328306
  13. Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences.
    Nature. 1984 Jun 28-Jul 4;309(5971):757-62 PMID: 6330561
  14. The T cell receptor beta chain genes are located on chromosome 6 in mice and chromosome 7 in humans.
    Cell. 1984 Jul;37(3):1091-9 PMID: 6331676
  15. Genomic organization and sequence of T-cell receptor beta-chain constant- and joining-region genes.
    Nature. 1984 Aug 2-8;310(5976):387-91 PMID: 6336329
  16. Molecular basis of a mouse strain-specific anti-hapten response.
    Cell. 1983 May;33(1):85-93 PMID: 6432337
  17. The major histocompatibility complex-restricted antigen receptor on T cells: distribution on thymus and peripheral T cells.
    Cell. 1984 Sep;38(2):577-84 PMID: 6331891
  18. The structure, rearrangement and expression of D beta gene segments of the murine T-cell antigen receptor.
    Nature. 1984 Sep 27-Oct 3;311(5984):344-50 PMID: 6090932
  19. Structure, expression and divergence of T-cell receptor beta-chain variable regions.
    Nature. 1984 Nov 1-7;312(5989):40-6 PMID: 6092964
  20. Sequence and expression of transcripts of the human T-cell receptor beta-chain genes.
    Nature. 1984 Dec 6-12;312(5994):521-4 PMID: 6334237
  21. Rearranged beta T cell receptor genes in a helper T cell clone specific for lysozyme: no correlation between V beta and MHC restriction.
    Cell. 1985 Apr;40(4):859-67 PMID: 2580639
  22. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.
    J Immunol. 1985 Jun;134(6):3994-4000 PMID: 2580901
  23. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  24. Antibody diversity.
    Science. 1978 Oct 6;202(4363):11-7 PMID: 99815
  25. Polymorphism of germ-line immunoglobulin VH genes correlates with allotype and idiotype markers.
    Eur J Immunol. 1981 Dec;11(12):1017-20 PMID: 6276186
  26. Polymorphism in anti-phosphocholine antibodies reflecting evolution of immunoglobulin families.
    J Exp Med. 1983 Jan 1;157(1):98-113 PMID: 6401319
  27. A highly homologous T-cell receptor beta-chain variable region is expressed in mouse and human T cells.
    Immunogenetics. 1985;21(4):403-6 PMID: 3873411
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.3.767
Published
1986-02-00
Pages
767-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322946
Subset
IM
Grants
NIGMS NIH HHS · GM07200 · United States
Databases
GENBANK
M11858, M11859, M11860
External Links
PubMed source DOI: 10.1073/pnas.83.3.767 PMC Full Text View journal details
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