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PMID: 3462701 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6382-6

Sive HL, Roeder RG

Abstract

We have examined the interaction of factors in HeLa cell nuclear extracts with a human histone H2B gene (H2B) promoter. Protein-DNA mobility-shift and DNase I protection assays detected a factor(s) binding to a 15-base-pair consensus element that is essential for efficient H2B transcription in vitro. Part of this consensus sequence is the octanucleotide ATTTGCAT, which is apparently a functional component of several non-histone genes. A subset of these genes, including a human U2 small nuclear RNA (snRNA) gene promoter, a mouse immunoglobulin heavy chain enhancer, and a mouse light chain promoter, were shown to interact with the H2B consensus sequence-binding factor(s). These results suggest that a common factor or closely related factors may contribute to the regulation of these and other genes that share the octanucleotide sequence.

MeSH Terms
Base Sequence Binding, Competitive Cell Nucleus/physiology DNA-Binding Proteins/physiology Enhancer Elements, Genetic Genes, Regulator HeLa Cells Histones/genetics Humans Immunoglobulins/genetics Promoter Regions, Genetic RNA, Small Nuclear/genetics Transcription Factors/physiology
Chemicals
DNA-Binding Proteins Histones Immunoglobulins RNA, Small Nuclear Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sive H L
Roeder R G
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6382
Published
1986-09-00
Pages
6382-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386507
Subset
IM
Grants
NCI NIH HHS · CA34223 · United States
NCI NIH HHS · CA34891 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.17.6382 PMC Full Text View journal details
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