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PMID: 6438631 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two regulatory elements for immunoglobulin kappa light chain gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 22 ·1984-11-00 ·Pages 7041-5

Bergman Y, Rice D, Grosschedl R, Baltimore D

Abstract

By using internal deletions within a rearranged immunoglobulin kappa light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the "TATA" box, between positions -69 and -104. These two elements both are required for efficient kappa chain gene expression.

MeSH Terms
Animals Base Sequence Chromosome Mapping Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Mice Promoter Regions, Genetic RNA, Messenger/genetics
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin kappa-Chains RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bergman Y
Rice D
Grosschedl R
Baltimore D
References (28)
28 references, click to expand
  1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  2. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  3. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  4. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  5. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6 PMID: 6938957
  6. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  7. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  8. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  9. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
    Cell. 1981 Dec;27(2 Pt 1):299-308 PMID: 6277502
  10. Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes.
    Cell. 1981 Dec;27(2 Pt 1):381-90 PMID: 6277505
  11. Efficient infection of monkey cells with DNA of simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
  12. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  13. Chromatin changes accompany immunoglobulin kappa gene activation: a potential control region within the gene.
    Nature. 1982 Sep 30;299(5882):449-51 PMID: 6811947
  14. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
    J Mol Appl Genet. 1982;1(5):457-81 PMID: 6296253
  15. Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7862-5 PMID: 6818555
  16. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  17. DNase I-hypersensitive sites in the chromatin of immunoglobulin kappa light chain genes.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2427-31 PMID: 6221340
  18. Variable amplification of immunoglobulin lambda light-chain genes in human populations.
    Nature. 1983 Jul 14-20;304(5922):172-4 PMID: 6306474
  19. Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus.
    Science. 1983 Aug 12;221(4611):663-5 PMID: 6306772
  20. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  21. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  22. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  23. Biosynthesis, glycosylation, and in vitro translation of the human T cell antigen Leu-4.
    J Immunol. 1983 Oct;131(4):1876-81 PMID: 6413583
  24. Cell-type specific expression of a transfected immunoglobulin gene.
    Nature. 1983 Nov 3-9;306(5938):77-9 PMID: 6415486
  25. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  26. Fine mapping of an immunoglobulin gene activator.
    Mol Cell Biol. 1984 Jun;4(6):1042-9 PMID: 6330526
  27. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  28. A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene.
    Nature. 1984 Jan 5-11;307(5946):80-2 PMID: 6419128
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.22.7041
Published
1984-11-00
Pages
7041-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392072
Subset
IM
Databases
GENBANK
K02794
External Links
PubMed source DOI: 10.1073/pnas.81.22.7041 PMC Full Text View journal details
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