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PMID: 15208397 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana.

The Plant cell ·Vol. 16 ·No. 7 ·2004-07-00 ·Pages 1898-911

Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ, Masson PH, Murphy AS

Abstract

Aglycone flavonols are thought to modulate auxin transport in Arabidopsis thaliana via an as yet undefined mechanism. Biochemical studies suggest that flavonoids interact with regulatory proteins rather than directly with the PIN auxin efflux facilitator proteins. Auxin transport is enhanced in the absence of flavonoids (transparent testa4 [tt4]) and reduced in the presence of excess flavonols (tt7 and tt3). Steady state PIN mRNA levels in roots inversely correlate with auxin movement in tt mutants. PIN gene transcription and protein localization in flavonoid-deficient mutants appear to be modulated by developmental cues and are auxin responsive. Modulation of PIN gene expression and protein distribution by localized auxin accumulations occurs in the wild type as well. Flavonoids inhibit auxin transport primarily at the shoot apex and root tip and appear to modulate vesicular cycling of PIN1 at the root tip. In some auxin-accumulating tissues, flavonoid increases and changes in flavonoid speciation are subsequent to auxin accumulation.

Keywords
NASA Discipline Plant Biology Non-NASA Center
MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Biological Transport Carrier Proteins/genetics,metabolism Flavonoids/metabolism Gene Expression Regulation, Plant Immunohistochemistry Indoleacetic Acids/metabolism Membrane Proteins/genetics,metabolism Membrane Transport Proteins Mutation Plant Roots/growth & development,metabolism Plant Shoots/growth & development,metabolism Recombinant Fusion Proteins/genetics,metabolism Seeds/drug effects,growth & development
Chemicals
Arabidopsis Proteins Carrier Proteins Flavonoids Indoleacetic Acids Membrane Proteins Membrane Transport Proteins PIN1 protein, Arabidopsis Recombinant Fusion Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Peer Wendy Ann
Department of Horticulture, Purdue University, West Lafayette, Indiana 47907-2010, USA. [email protected]
Bandyopadhyay Anindita
Blakeslee Joshua J
Makam Srinivas N
Chen Rujin J
Masson Patrick H
Murphy Angus S
Investigators
1 investigators, click to expand
Masson P H
U WI, Madison
References (65)
65 references, click to expand
  1. Small molecule modulators of cyclin-dependent kinases for cancer therapy.
    Oncogene. 2000 Dec 27;19(56):6600-6 PMID: 11426645
  2. Genetic and chemical reductions in protein phosphatase activity alter auxin transport, gravity response, and lateral root growth.
    Plant Cell. 2001 Jul;13(7):1683-97 PMID: 11449059
  3. AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis.
    Cell. 2002 Mar 8;108(5):661-73 PMID: 11893337
  4. An emerging model of auxin transport regulation.
    Plant Cell. 2002 Feb;14(2):293-9 PMID: 11884675
  5. Modulation by (iso)flavonoids of the ATPase activity of the multidrug resistance protein.
    FEBS Lett. 1997 Aug 18;413(2):344-8 PMID: 9280310
  6. The effect of quercetin on the phosphorylation activity of the Rous sarcoma virus transforming gene product in vitro and in vivo.
    Eur J Biochem. 1983 Oct 3;135(3):583-9 PMID: 6311542
  7. AtPIN2 defines a locus of Arabidopsis for root gravitropism control.
    EMBO J. 1998 Dec 1;17(23):6903-11 PMID: 9843496
  8. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis.
    Plant Physiol. 2001 Jun;126(2):524-35 PMID: 11402184
  9. Auxin transport inhibitors block PIN1 cycling and vesicle trafficking.
    Nature. 2001 Sep 27;413(6854):425-8 PMID: 11574889
  10. Pollination- or Wound-Induced Kaempferol Accumulation in Petunia Stigmas Enhances Seed Production.
    Plant Cell. 1994 Jan;6(1):11-23 PMID: 12244218
  11. Relationship between flavonoid structure and inhibition of phosphatidylinositol 3-kinase: a comparison with tyrosine kinase and protein kinase C inhibition.
    Biochem Pharmacol. 1997 Jun 1;53(11):1649-57 PMID: 9264317
  12. The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth.
    Cell. 2003 Jan 24;112(2):219-30 PMID: 12553910
  13. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
    Methods. 2001 Dec;25(4):402-8 PMID: 11846609
  14. Relocalization of the PIN1 auxin efflux facilitator plays a role in phototropic responses.
    Plant Physiol. 2004 Jan;134(1):28-31 PMID: 14730061
  15. Naturally occurring auxin transport regulators.
    Science. 1988 Jul 15;241(4863):346-9 PMID: 17734864
  16. Modulation by flavonoids of cell multidrug resistance mediated by P-glycoprotein and related ABC transporters.
    Cell Mol Life Sci. 2002 Feb;59(2):307-22 PMID: 11915946
  17. Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth.
    Plant J. 2001 Nov;28(4):465-74 PMID: 11737783
  18. ALTERED RESPONSE TO GRAVITY is a peripheral membrane protein that modulates gravity-induced cytoplasmic alkalinization and lateral auxin transport in plant statocytes.
    Plant Cell. 2003 Nov;15(11):2612-25 PMID: 14507996
  19. The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsis npq and tt mutants.
    Planta. 2001 Oct;213(6):953-66 PMID: 11722132
  20. Nitrogen fixation. Endocrine disrupters and flavonoid signalling.
    Nature. 2001 Sep 13;413(6852):128-9 PMID: 11557969
  21. Regulation of phyllotaxis by polar auxin transport.
    Nature. 2003 Nov 20;426(6964):255-60 PMID: 14628043
  22. TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19.
    Mol Biol Cell. 2003 Oct;14(10):4238-49 PMID: 14517332
  23. Identification, purification, and molecular cloning of N-1-naphthylphthalmic acid-binding plasma membrane-associated aminopeptidases from Arabidopsis.
    Plant Physiol. 2002 Mar;128(3):935-50 PMID: 11891249
  24. Modulation of pro-survival Akt/protein kinase B and ERK1/2 signaling cascades by quercetin and its in vivo metabolites underlie their action on neuronal viability.
    J Biol Chem. 2003 Sep 12;278(37):34783-93 PMID: 12826665
  25. Transporters on demand: intrahepatic pools of canalicular ATP binding cassette transporters in rat liver.
    J Biol Chem. 2001 Mar 9;276(10):7218-24 PMID: 11113123
  26. Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions.
    Genetics. 2003 Apr;163(4):1449-55 PMID: 12702688
  27. The TRANSPARENT TESTA16 locus encodes the ARABIDOPSIS BSISTER MADS domain protein and is required for proper development and pigmentation of the seed coat.
    Plant Cell. 2002 Oct;14(10):2463-79 PMID: 12368498
  28. Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis.
    Nature. 2002 Feb 14;415(6873):806-9 PMID: 11845211
  29. The TT8 gene encodes a basic helix-loop-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques.
    Plant Cell. 2000 Oct;12(10):1863-78 PMID: 11041882
  30. Tyrosine protein kinase activity in the DMBA-induced rat mammary tumor: inhibition by quercetin.
    Biochem Biophys Res Commun. 1984 Sep 28;123(3):1227-33 PMID: 6091650
  31. Specific inhibitor of puromycin-sensitive aminopeptidase with a homophthalimide skeleton: identification of the target molecule and a structure-activity relationship study.
    Bioorg Med Chem. 2001 Jan;9(1):121-31 PMID: 11197332
  32. A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis.
    EMBO J. 1996 May 1;15(9):2115-24 PMID: 8641277
  33. Inhibition of the calcium- and phospholipid-dependent protein kinase activity from mouse brain cytosol by quercetin.
    Biochem Biophys Res Commun. 1983 Dec 16;117(2):444-7 PMID: 6661236
  34. Local, efflux-dependent auxin gradients as a common module for plant organ formation.
    Cell. 2003 Nov 26;115(5):591-602 PMID: 14651850
  35. The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed.
    Plant Cell. 2001 Sep;13(9):2099-114 PMID: 11549766
  36. A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis.
    Plant Physiol. 1988 Mar;86(3):822-5 PMID: 16665995
  37. The arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):15112-7 PMID: 9844024
  38. AUX1 promotes lateral root formation by facilitating indole-3-acetic acid distribution between sink and source tissues in the Arabidopsis seedling.
    Plant Cell. 2002 Mar;14(3):589-97 PMID: 11910006
  39. Regulation of auxin response by the protein kinase PINOID.
    Cell. 2000 Feb 18;100(4):469-78 PMID: 10693763
  40. Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis.
    Plant J. 1995 Nov;8(5):659-71 PMID: 8528278
  41. Vesicular cycling mechanisms that control auxin transport polarity.
    Trends Plant Sci. 2003 Jul;8(7):301-4 PMID: 12878008
  42. Regulation of auxin transport by aminopeptidases and endogenous flavonoids.
    Planta. 2000 Aug;211(3):315-24 PMID: 10987549
  43. Auxin Transport Inhibitors: IV. EVIDENCE OF A COMMON MODE OF ACTION FOR A PROPOSED CLASS OF AUXIN TRANSPORT INHIBITORS: THE PHYTOTROPINS.
    Plant Physiol. 1980 Dec;66(6):1190-5 PMID: 16661601
  44. Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants.
    Science. 2003 Oct 3;302(5642):81-4 PMID: 14526073
  45. Auxin transport is required for hypocotyl elongation in light-grown but not dark-grown Arabidopsis.
    Plant Physiol. 1998 Feb;116(2):455-62 PMID: 9489005
  46. The PINOID protein kinase regulates organ development in Arabidopsis by enhancing polar auxin transport.
    Development. 2001 Oct;128(20):4057-67 PMID: 11641228
  47. The N-1-Naphthylphthalamic Acid-Binding Protein Is an Integral Membrane Protein.
    Plant Physiol. 1996 Jun;111(2):427-432 PMID: 12226298
  48. Flavonoid accumulation patterns of transparent testa mutants of arabidopsis.
    Plant Physiol. 2001 Jun;126(2):536-48 PMID: 11402185
  49. PINOID-mediated signaling involves calcium-binding proteins.
    Plant Physiol. 2003 Jul;132(3):1623-30 PMID: 12857841
  50. Flavonol-stimulated efflux of 7,12-dimethylbenz(a)anthracene in multidrug-resistant breast cancer cells.
    Cancer Res. 1993 Dec 15;53(24):5977-81 PMID: 7903198
  51. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue.
    Science. 1998 Dec 18;282(5397):2226-30 PMID: 9856939
  52. The transparent testa4 mutation prevents flavonoid synthesis and alters auxin transport and the response of Arabidopsis roots to gravity and light.
    Plant Cell. 2004 May;16(5):1191-205 PMID: 15100399
  53. Flavonoids and UV photoprotection in Arabidopsis mutants.
    Z Naturforsch C. 2001 Sep-Oct;56(9-10):745-54 PMID: 11724379
  54. Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF.
    Science. 1999 Oct 8;286(5438):316-8 PMID: 10514379
  55. Flavonoids induced in cells undergoing nodule organogenesis in white clover are regulators of auxin breakdown by peroxidase.
    J Exp Bot. 2001 Mar;52(Spec Issue):419-26 PMID: 11326048
  56. Phytotropin-binding sites and auxin transport in Cucurbita pepo: evidence for two recognition sites.
    Planta. 1992 May;187(2):254-60 PMID: 24178053
  57. Active auxin uptake by zucchini membrane vesicles: quantitation using ESR volume and delta pH determinations.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6541-5 PMID: 2995970
  58. Protein phosphorylation in the delivery of and response to auxin signals.
    Plant Mol Biol. 2002 Jun-Jul;49(3-4):285-303 PMID: 12036255
  59. Enhanced gravi- and phototropism in plant mdr mutants mislocalizing the auxin efflux protein PIN1.
    Nature. 2003 Jun 26;423(6943):999-1002 PMID: 12827205
  60. Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development.
    Plant Cell. 2001 Nov;13(11):2441-54 PMID: 11701880
  61. A New Vertical Mesh Transfer Technique for Metal-Tolerance Studies in Arabidopsis (Ecotypic Variation and Copper-Sensitive Mutants).
    Plant Physiol. 1995 May;108(1):29-38 PMID: 12228451
  62. Evidence for altered ion transport in Saccharomyces cerevisiae overexpressing human MDR 1 protein.
    Biochemistry. 1999 Mar 30;38(13):4214-26 PMID: 10194338
  63. Quercetin inhibits the expression and function of the androgen receptor in LNCaP prostate cancer cells.
    Carcinogenesis. 2001 Mar;22(3):409-14 PMID: 11238180
  64. Dietary flavonols quercetin and kaempferol are ligands of the aryl hydrocarbon receptor that affect CYP1A1 transcription differentially.
    Biochem J. 1999 Jun 15;340 ( Pt 3):715-22 PMID: 10359656
  65. The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium.
    Plant Cell. 2001 Apr;13(4):853-71 PMID: 11283341
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.021501
Email
[email protected]
Published
2004-07-00
Epub
2004-00-18
Pages
1898-911
Language
English
Region
England
NLM ID
9208688
PMCID
PMC514169
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.021501 PMC Full Text View journal details
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