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PMID: 15249690 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trim5alpha protein restricts both HIV-1 and murine leukemia virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 29 ·2004-07-20 ·Pages 10786-91

Yap MW, Nisole S, Lynch C, Stoye JP

Abstract

Replication of HIV-1 and N-tropic murine leukemia virus (N-MLV) is restricted in a number of different primate cells. In some cell lines, cross-saturation experiments suggest that the two viruses are interacting with the same restriction factor. Recently, Trim5alpha protein from rhesus monkey was found to restrict HIV-1. We have confirmed this result and have shown that Trim5alpha from two African green monkey cell lines, Vero and CV-1, also restricts HIV-1. In addition, we show that human, rhesus, and African green monkey Trim5alpha can restrict N-MLV. By using a panel of MLV capsid mutants, subtle differences in the anti-MLV activity were identified among the different primate Trim5alpha cDNAs. Trim1 isolated from humans and green monkeys was also found to restrict N-MLV. We hypothesize that the Trim family of proteins plays a widespread role in innate immunity to viral infection.

MeSH Terms

Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Antiviral Restriction Factors Capsid Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Cell Line Chlorocebus aethiops HIV-1/physiology Humans Leukemia Virus, Murine/physiology Macaca mulatta Membrane Proteins/metabolism Mice Molecular Sequence Data Proteins/chemistry,genetics,metabolism RNA, Small Interfering/genetics,metabolism Sequence Alignment Tripartite Motif Proteins Ubiquitin-Protein Ligases Virus Replication

Chemicals

Adaptor Proteins, Signal Transducing Antiviral Restriction Factors Capsid Proteins Carrier Proteins Membrane Proteins Proteins RNA, Small Interfering TRAT1 protein, human Tripartite Motif Proteins TRIM5 protein, human TRIM5(alpha) protein, rhesus monkey Ubiquitin-Protein Ligases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yap Melvyn W

Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.

Nisole Sébastien

Lynch Clare

Stoye Jonathan P

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0402876101

Published

2004-07-20

Epub

2004-00-12

Pages

10786-91

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC490012

Subset

Databases

GENBANK

AY625000, AY625001, AY625002, AY625003, AY625004, AY625005, AY625006, AY625007

Corrections

CommentIn

PMID: 15252204

External Links

PubMed source DOI: 10.1073/pnas.0402876101 PMC Full Text View journal details

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Trim5alpha protein restricts both HIV-1 and murine leukemia virus.

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