Home LiteratureArticle Details
PMID: 1527167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.

The Journal of cell biology ·Vol. 119 ·No. 1 ·1992-10-00 ·Pages 17-25

Ulitzur N, Harel A, Feinstein N, Gruenbaum Y

Abstract

The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a Drosophila in vitro assembly system that reconstitutes nuclei from added sperm chromatin or naked DNA. Upon incubation of the embryonic assembly extract with anti-Drosophila lamin antibodies, the attachment of nuclear membrane vesicles to chromatin surface and nuclear envelope formation did not occur. Lamina assembly and nuclear membrane vesicles attachment to the chromatin were inhibited only when the activity of the 75-kD lamin isoform was inhibited in both soluble and membrane-vesicles fractions. Incubation of decondensed sperm chromatin with an extract that was depleted of nuclear membranes revealed the presence of lamin molecules on the chromatin periphery. In addition, high concentrations of bacterially expressed lamin molecules added to the extract, were able to associate with the chromatin periphery, and did not inhibit nuclear envelope assembly. After nuclear reconstitution, a fraction of the lamin pool was converted into the typical 74- and 76-kD isoforms. Together, these data strongly support an essential role of the lamina in nuclear envelope assembly.

MeSH Terms
Animals Cell Nucleus/metabolism,ultrastructure Cell-Free System Chromatin/metabolism DNA/metabolism Drosophila/metabolism Embryo, Nonmammalian/metabolism Fluorescent Antibody Technique Lamins Microscopy, Electron Nuclear Envelope/metabolism,ultrastructure Nuclear Proteins/metabolism
Chemicals
Chromatin Lamins Nuclear Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ulitzur N
Department of Genetics, Hebrew University of Jerusalem, Israel.
Harel A
Feinstein N
Gruenbaum Y
References (36)
36 references, click to expand
  1. Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts.
    Cell. 1987 Dec 24;51(6):1009-18 PMID: 3690659
  2. Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies.
    J Cell Biol. 1986 Nov;103(5):1847-54 PMID: 3536954
  3. A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
    Cell. 1986 Feb 28;44(4):639-52 PMID: 3948244
  4. Binding of lamin A to polynucleosomes.
    J Biol Chem. 1991 May 15;266(14):9211-5 PMID: 2026620
  5. Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin.
    J Cell Biol. 1991 Mar;112(6):1073-82 PMID: 1825658
  6. Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
    J Cell Biol. 1984 Apr;98(4):1222-30 PMID: 6609160
  7. Assembly of the cell nucleus.
    Trends Genet. 1990 Dec;6(12):406-10 PMID: 2087783
  8. Nuclear formation in a Drosophila cell-free system.
    Exp Cell Res. 1990 Nov;191(1):64-70 PMID: 2171967
  9. Lamins A and C bind and assemble at the surface of mitotic chromosomes.
    J Cell Biol. 1990 Sep;111(3):1047-57 PMID: 2202732
  10. Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
    Cell. 1990 Jan 12;60(1):17-29 PMID: 2295087
  11. Interconversion of Drosophila nuclear lamin isoforms during oogenesis, early embryogenesis, and upon entry of cultured cells into mitosis.
    J Cell Biol. 1989 Feb;108(2):255-65 PMID: 2492999
  12. A somatic cell-derived system for studying both early and late mitotic events in vitro.
    J Cell Sci. 1989 Nov;94 ( Pt 3):449-62 PMID: 2632578
  13. Dominoes and clocks: the union of two views of the cell cycle.
    Science. 1989 Nov 3;246(4930):614-21 PMID: 2683077
  14. Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene.
    J Cell Biol. 1988 Mar;106(3):585-96 PMID: 3126192
  15. A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
    J Cell Biol. 1988 Jul;107(1):57-68 PMID: 3392106
  16. Biosynthesis and interconversion of Drosophila nuclear lamin isoforms during normal growth and in response to heat shock.
    J Cell Biol. 1987 Aug;105(2):771-90 PMID: 3624309
  17. Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
    Cell. 1985 May;41(1):177-90 PMID: 3888407
  18. Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
    Science. 1983 May 13;220(4598):719-21 PMID: 6601299
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  20. DNA replication in cell-free extracts from Drosophila melanogaster.
    EMBO J. 1991 Dec;10(13):4361-9 PMID: 1756740
  21. Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro.
    Cell. 1991 Apr 19;65(2):209-17 PMID: 1849796
  22. Interaction of Xenopus lamins A and LII with chromatin in vitro mediated by a sequence element in the carboxyterminal domain.
    Exp Cell Res. 1991 Dec;197(2):280-9 PMID: 1959562
  23. Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
    Cell. 1991 May 17;65(4):569-78 PMID: 2032284
  24. The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs.
    J Cell Sci. 1991 Mar;98 ( Pt 3):271-9 PMID: 2055960
  25. Immunoaffinity purification and functional characterization of interphase and meiotic Drosophila nuclear lamin isoforms.
    J Biol Chem. 1990 Jul 25;265(21):12596-601 PMID: 2115522
  26. Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
    J Biol Chem. 1990 May 15;265(14):7808-13 PMID: 2186029
  27. Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery.
    Cell. 1990 Jul 13;62(1):89-106 PMID: 2194675
  28. A lamin-independent pathway for nuclear envelope assembly.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2247-59 PMID: 2277059
  29. On the cell-free association of lamins A and C with metaphase chromosomes.
    Exp Cell Res. 1990 Jan;186(1):169-76 PMID: 2298234
  30. Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos.
    J Cell Sci. 1989 Nov;94 ( Pt 3):463-70 PMID: 2517292
  31. Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos.
    FEBS Lett. 1989 Dec 18;259(1):113-6 PMID: 2557241
  32. Replication occurs at discrete foci spaced throughout nuclei replicating in vitro.
    J Cell Sci. 1989 Nov;94 ( Pt 3):471-7 PMID: 2632579
  33. A lamin B receptor in the nuclear envelope.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8531-4 PMID: 2847165
  34. Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
    Cell. 1987 Jan 30;48(2):205-17 PMID: 3026635
  35. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  36. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
    Cell. 1986 Nov 21;47(4):577-87 PMID: 3779837
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.1.17
Published
1992-10-00
Pages
17-25
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289631
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.119.1.17 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2