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PMID: 15272302 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

MAPKKKalpha is a positive regulator of cell death associated with both plant immunity and disease.

The EMBO journal ·Vol. 23 ·No. 15 ·2004-08-04 ·Pages 3072-82

del Pozo O, Pedley KF, Martin GB

Abstract

Many plant pathogens cause disease symptoms that manifest over days as regions of localized cell death. Localized cell death (the hypersensitive response; HR) also occurs in disease-resistant plants, but this response appears within hours of attempted infection and may restrict further pathogen growth. We identified a MAP kinase kinase kinase gene (MAPKKKalpha) that is required for the HR and resistance against Pseudomonas syringae. Significantly, we found that MAPKKKalpha also regulates cell death in susceptible leaves undergoing P. syringae infection. Overexpression of MAPKKKalpha in leaves activated MAPKs and caused pathogen-independent cell death. By overexpressing MAPKKKalpha in leaves and suppressing expression of various MAPKK and MAPK genes by virus-induced gene silencing, we identified two distinct MAPK cascades that act downstream of MAPKKKalpha. These results demonstrate that signal transduction pathways associated with both plant immunity and disease susceptibility share a common molecular switch.

MeSH Terms
Amino Acid Sequence Cell Death Disease Susceptibility Enzyme Activation Gene Expression Regulation, Plant MAP Kinase Kinase Kinases/chemistry,genetics,metabolism MAP Kinase Signaling System Mitogen-Activated Protein Kinase Kinases/metabolism Molecular Sequence Data Phylogeny Plant Diseases/microbiology Plant Leaves/cytology,immunology,metabolism,microbiology Pseudomonas syringae/physiology Sequence Alignment Tobacco/cytology,immunology,metabolism,microbiology
Chemicals
MAP Kinase Kinase Kinases Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
del Pozo Olga
Boyce Thompson Institute for Plant Research, Ithaca, NY, USA.
Pedley Kerry F
Martin Gregory B
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32 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600283
Published
2004-08-04
Epub
2004-00-22
Pages
3072-82
Language
English
Region
England
NLM ID
8208664
PMCID
PMC514913
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600283 PMC Full Text View journal details
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