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PMID: 14592968 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.

The EMBO journal ·Vol. 22 ·No. 21 ·2003-11-03 ·Pages 5690-9

Lu R, Malcuit I, Moffett P, Ruiz MT, Peart J, Wu AJ, Rathjen JP, Bendahmane A, Day L, Baulcombe DC

Abstract

Virus-induced gene silencing was used to assess the function of random Nicotiana benthamiana cDNAs in disease resistance. Out of 4992 cDNAs tested from a normalized library, there were 79 that suppressed a hypersensitive response (HR) associated with Pto-mediated resistance against Pseudomonas syringae. However, only six of these clones blocked the Pto-mediated suppression of P.syringae growth. The three clones giving the strongest loss of Pto resistance had inserts corresponding to HSP90 and also caused loss of Rx-mediated resistance against potato virus X and N-mediated tobacco mosaic virus resistance. The role of HSP90 as a cofactor of disease resistance is associated with stabilization of Rx protein levels and could be accounted for in part by SGT1 and other cofactors of disease resistance acting as co-chaperones. This approach illustrates the potential benefits and limitations of RNA silencing in forward screens of gene function in plants.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Complementary/genetics DNA, Plant/genetics Gene Silencing Genes, Plant HSP90 Heat-Shock Proteins/genetics Molecular Sequence Data Plant Diseases/genetics,microbiology,virology Plant Proteins/genetics Potexvirus/pathogenicity Protein Serine-Threonine Kinases/physiology Pseudomonas syringae/pathogenicity Sequence Homology, Amino Acid Tobacco/genetics,microbiology,virology Tobacco Mosaic Virus/pathogenicity
Chemicals
DNA, Complementary DNA, Plant HSP90 Heat-Shock Proteins Plant Proteins Pto protein, Lycopersicon Protein Serine-Threonine Kinases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Lu Rui
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Malcuit Isabelle
Moffett Peter
Ruiz Maria T
Peart Jack
Wu Ai-Jiuan
Rathjen John P
Bendahmane Abdelhafid
Day Louise
Baulcombe David C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg546
Published
2003-11-03
Pages
5690-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC275403
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg546 PMC Full Text View journal details
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