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PMID: 9426614 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genomic organization of hsp90 gene family in Arabidopsis.

Plant molecular biology ·Vol. 35 ·No. 6 ·1997-12-00 ·Pages 955-61

Milioni D, Hatzopoulos P

Abstract

We have isolated six members of the hsp90 gene family from Arabidopsis thaliana. Three genes designated hsp81.2, 81.3 and 81.4 are clustered within a 15 kb genomic region while two of these are 1.5 kb apart in a head-to-head orientation. The deduced amino acid sequence shows that the members can be divided into two types. The hsp81.1, 81.2, 81.3 and 81.4 genes comprise the cytosolic hsp90 type having few introns. However, the hsp88.1 and 89.1 genes comprising the organelle type are composed of 18 or 19 introns. Sequence comparison showed there is high homology among the cytosolic members while there is less homology among the organelle members. The expression of the hsp90 genes and mRNA accumulation in plants and calli is very low at control temperatures and is strongly induced by heat-shock. Arsenite stress strongly stimulates the expression of this gene family.

MeSH Terms
Amino Acid Sequence Arabidopsis/chemistry,genetics Chromosome Mapping Cloning, Molecular Gene Expression Regulation, Plant/drug effects Genome, Plant HSP90 Heat-Shock Proteins/biosynthesis,genetics Hot Temperature Metals, Heavy/pharmacology Molecular Sequence Data Multigene Family Plant Proteins/biosynthesis,genetics Sequence Analysis, DNA
Chemicals
HSP90 Heat-Shock Proteins Metals, Heavy Plant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Milioni D
Department of Agricultural Biology and Biotechnology, Agricultural University of Athens, Greece.
Hatzopoulos P
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28 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005874521528
Published
1997-12-00
Pages
955-61
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
Y07613
External Links
PubMed source DOI: 10.1023/a:1005874521528 View journal details
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