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PMID: 7764623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heat-inducible rice hsp82 and hsp70 are not always co-regulated.

Planta ·Vol. 193 ·No. 1 ·1994-00-00 ·Pages 57-66

Van Breusegem F, Dekeyser R, Garcia AB, Claes B, Gielen J, Van Montagu M, Caplan AB

Abstract

We have characterized several heat-shock-induced genes in rice (Oryza sativa L.) and compared their expression under a variety of conditions. Three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. The middle member of this cluster, designated hsp82B, has been fully sequenced. The gene uses a promoter containing six putative heat-shock elements as well as several unusual sequence motifs including a stretch of 11 thymidines alternating with 11 adenosines. The mRNA for this gene reaches its highest relative level of expression within 120 min after plants are shifted to 42 degrees C; no other conditions induce this gene. By contrast, we found that during heat stress the expression of hsp70 correlates well with increases in internal ion concentrations, and can also be induced by excess salt or ethanol at normal growth temperatures. These results appear to indicate that whereas hsp70 is induced by all stresses that lead to protein denaturation-including heat stress-HSP82 mRNA accumulates only upon heat stress.

Related Genes
hsp70 hsp82 hsp82B
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Fungal Proteins/genetics Gene Expression Regulation Genes, Plant HSP90 Heat-Shock Proteins Heat-Shock Proteins/genetics Hot Temperature Humans Molecular Sequence Data Multigene Family Oryza/genetics Plant Proteins/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Fungal Proteins HSP82 protein, S cerevisiae HSP90 Heat-Shock Proteins Heat-Shock Proteins Plant Proteins Saccharomyces cerevisiae Proteins DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Van Breusegem F
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Dekeyser R
Garcia A B
Claes B
Gielen J
Van Montagu M
Caplan A B
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/BF00191607
Published
1994-00-00
Pages
57-66
Language
English
Region
Germany
NLM ID
1250576
Databases
GENBANK
Z11920, Z15018, Z15024
External Links
PubMed source DOI: 10.1007/BF00191607 View journal details
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