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PMID: 2779571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Three light-inducible heat shock genes of Chlamydomonas reinhardtii.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 3911-8

von Gromoff ED, Treier U, Beck CF

Abstract

Genomic clones representing three Chlamydomonas reinhardtii genes homologous to the Drosophila hsp70 heat shock gene were isolated. The mRNAs of genes hsp68, hsp70, and hsp80 could be translated in vitro into proteins of Mr 68,000, 70,000, and 80,000, respectively. Transcription of these genes increased dramatically upon heat shock, and the corresponding mRNAs rapidly accumulated, reaching a peak at around 30 min after a shift to the elevated temperature. Light also induced the accumulation of the mRNAs encoded by these heat shock genes. A shift of dark-grown cells to light resulted in a drastic increase in mRNA levels, which reached a maximum at around 1 h after the shift. Thus, in Chlamydomonas, expression of hsp70-homologous heat shock genes appears to be regulated by thermal stress and light.

MeSH Terms
Chlamydia/genetics Cloning, Molecular DNA, Bacterial/genetics Gene Expression Regulation/radiation effects Genes, Bacterial Heat-Shock Proteins/genetics Hot Temperature Light RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
DNA, Bacterial Heat-Shock Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
von Gromoff E D
Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Federal Republic of Germany.
Treier U
Beck C F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.3911-3918.1989
Published
1989-09-00
Pages
3911-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362453
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.9.3911-3918.1989 PMC Full Text View journal details
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