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PMID: 15277213 Published · ppublish English Journal Article

Degranulation of paneth cells via toll-like receptor 9.

The American journal of pathology ·Vol. 165 ·No. 2 ·2004-08-00 ·Pages 373-81

Rumio C, Besusso D, Palazzo M, Selleri S, Sfondrini L, Dubini F, Ménard S, Balsari A

Abstract

The release of antimicrobial peptides and growth factors by Paneth cells is thought to play an important role in protecting the small intestine, but the mechanisms involved have remained obscure. Immunohistochemistry and immunofluorescence showed that Paneth cells express Toll-like receptor 9 (TLR9) in the granules. Injection of mice with oligonucleotides containing CpG sequence (CpG-ODNs) led to a down-modulation of TLR9 and a striking decrease in the number of large secretory granules, consistent with degranulation. Moreover CpG-ODN treatment increased resistance to oral challenge with virulent Salmonella typhimurium. Moreover, our findings demonstrate a sentinel role for Paneth cells through TLR9.

MeSH Terms
Adjuvants, Immunologic/pharmacology Animals Cells, Cultured Colorectal Neoplasms/metabolism,pathology Cytoplasmic Granules/drug effects,metabolism,pathology DNA-Binding Proteins/metabolism Fluorescent Antibody Technique Humans Immunoenzyme Techniques Intestine, Small/drug effects,metabolism,microbiology Mice Mice, Inbred C57BL Oligodeoxyribonucleotides/pharmacology Paneth Cells/drug effects,metabolism,microbiology Receptors, Cell Surface/metabolism Salmonella Infections, Animal/metabolism,pathology Salmonella typhimurium/pathogenicity Secretory Vesicles/drug effects,metabolism,pathology Survival Rate Toll-Like Receptor 9
Chemicals
Adjuvants, Immunologic CPG-oligonucleotide DNA-Binding Proteins Oligodeoxyribonucleotides Receptors, Cell Surface TLR9 protein, human Tlr9 protein, mouse Toll-Like Receptor 9
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Rumio Cristiano
Department of Human Morphology, University of Milan, Italy. [email protected].
Besusso Dario
Palazzo Marco
Selleri Silvia
Sfondrini Lucia
Dubini Francesco
Ménard Sylvie
Balsari Andrea
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)63304-4
Email
[email protected]
Published
2004-08-00
Pages
373-81
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1618569
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)63304-4 PMC Full Text View journal details
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