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PMID: 1530887 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

U1 and U2 small nuclear RNAs are present in nuclear speckles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 1 ·1992-01-01 ·Pages 305-8

Huang S, Spector DL

Abstract

The localization of U1 and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2'-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution, in addition to being present in three of four foci, in HeLa cell nuclei. However, in cells of defined passage, such as Detroit 551 and WI-38 fibroblasts, these snRNAs are concentrated in nuclear speckles, and foci are not observed. The speckled distribution of U1 and U2 snRNAs is coincident with the speckled regions enriched in small nuclear ribonucleoprotein particle (snRNP) proteins and the essential non-snRNP splicing factor SC-35. The localization of these key components of the pre-mRNA splicing machinery to speckled nuclear regions suggests that these regions may be involved in pre-mRNA splicing.

MeSH Terms

Cell Compartmentation Cell Nucleus/ultrastructure Fluorescent Antibody Technique HeLa Cells Humans In Vitro Techniques Nuclear Proteins/metabolism Nucleic Acid Hybridization RNA Splicing RNA, Small Nuclear/metabolism Ribonucleoproteins/immunology,metabolism Ribonucleoproteins, Small Nuclear

Chemicals

Nuclear Proteins RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Huang S

Cold Spring Harbor Laboratory, NY 11724.

Spector D L

References (22)

22 references, click to expand

Immunocytochemical identification of nuclear structures containing snRNPs in isolated rat liver cells.
J Ultrastruct Res. 1984 May;87(2):180-9 PMID: 6544870
Ultrastructural localization of transcription sites and of RNA distribution during the cell cycle of synchronized CHO cells.
Exp Cell Res. 1978 May;113(2):327-37 PMID: 95675
In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells.
EMBO J. 1991 Jul;10(7):1863-73 PMID: 1710980
Localization of pre-messenger RNA at discrete nuclear sites.
Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7391-5 PMID: 1831271
Association between the nucleolus and the coiled body.
J Struct Biol. 1990 Jul-Sep;104(1-3):120-7 PMID: 2088441
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
Nature. 1990 Feb 1;343(6257):437-41 PMID: 2137203
Intranuclear localization of snRNP antigens.
J Cell Biol. 1986 Jan;102(1):137-44 PMID: 2934400
Distribution of the 70K U1 RNA-associated protein during interphase and mitosis. Correlation with other U RNP particles and proteins of the nuclear matrix.
J Cell Sci. 1986 Dec;86:173-90 PMID: 2958489
5'-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Double-label immunofluorescent studies with anti-m3G antibodies and with anti-RNP and anti-Sm autoantibodies.
Exp Cell Res. 1984 Oct;154(2):548-60 PMID: 6207038
Ultrastructural distribution of nuclear ribonucleoproteins as visualized by immunocytochemistry on thin sections.
J Cell Biol. 1984 Jan;98(1):358-63 PMID: 6231300
Localization and characterization of newly synthesized nuclear RNA in isolate rat hepatocytes.
Exp Cell Res. 1976 Apr;99(1):155-64 PMID: 944140
Ultrastructural organization and biochemical characterization of chromatin - RNA - protein complexes isolated from mammalian cell nuclei.
Eur J Biochem. 1975 Oct 15;58(2):327-37 PMID: 1102303
Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.
EMBO J. 1991 Jan;10(1):195-206 PMID: 1824936
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
J Cell Biol. 1991 May;113(3):465-83 PMID: 1826687
Associations between distinct pre-mRNA splicing components and the cell nucleus.
EMBO J. 1991 Nov;10(11):3467-81 PMID: 1833187
Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles.
Proc Natl Acad Sci U S A. 1990 Jan;87(1):147-51 PMID: 2136950
Protein composition of mammalian spliceosomes assembled in vitro.
Proc Natl Acad Sci U S A. 1990 Oct;87(20):8031-5 PMID: 2146679
Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies.
J Immunol. 1989 Oct 15;143(8):2560-6 PMID: 2477448
Redistribution of U-snRNPs during mitosis.
Exp Cell Res. 1986 Mar;163(1):87-94 PMID: 2935418
Localisation of rapidly and slowly labelled nuclear RNA as visualized by high resolution autoradiography.
Exp Cell Res. 1971 Jul;67(1):129-41 PMID: 4105558
Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry.
Biol Cell. 1984;51(1):109-12 PMID: 6207877
Immunoelectron microscopic localization of snRNPs.
Biol Cell. 1983;49(1):1-10 PMID: 6230127

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.89.1.305

Published

1992-01-01

Pages

305-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC48225

Subset

Grants

NIGMS NIH HHS · R01 GM042694 · United States

NCI NIH HHS · 5P30 CA45508-03 · United States

NIGMS NIH HHS · GM42694 · United States

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1073/pnas.89.1.305 PMC Full Text View journal details

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U1 and U2 small nuclear RNAs are present in nuclear speckles.

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