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PMID: 1530959 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

T cell receptor complexes containing Fc epsilon RI gamma homodimers in lieu of CD3 zeta and CD3 eta components: a novel isoform expressed on large granular lymphocytes.

The Journal of experimental medicine ·Vol. 175 ·No. 1 ·1992-01-01 ·Pages 203-9

Koyasu S, D'Adamio L, Arulanandam AR, Abraham S, Clayton LK, Reinherz EL

Abstract

CD3 zeta and CD3 eta form disulfide-linked homo- or heterodimers important in targeting partially assembled Ti alpha-beta/CD3 gamma delta epsilon T cell receptor (TCR) complexes to the cell surface and transducing stimulatory signals after antigen recognition. Here we identify a new TCR isoform expressed on splenic CD2+, CD3/Ti alpha-beta+, CD4-, CD8-, CD16+, NK1.1+ mouse large granular lymphocytes (LGL), which are devoid of CD3 zeta and CD3 eta proteins. The TCRs of this subset contain homodimers of the gamma subunit of the high affinity receptor for IgE (Fc epsilon RI gamma) in lieu of CD3 zeta and/or CD3 eta proteins. The LGL display natural killer-like activity and are cytotoxic for B cell hybridomas producing anti-CD3 epsilon and anti-CD16 monoclonal antibodies, demonstrating the signaling capacity of both TCR and CD16 in this cell type. These findings provide evidence for an additional level of complexity of TCR signal transduction isoforms in naturally occurring T cell subsets.

MeSH Terms
Animals Antigens, Differentiation, B-Lymphocyte/genetics Antigens, Differentiation, T-Lymphocyte/genetics CD3 Complex Cell Line Cells, Cultured Cytotoxicity, Immunologic Flow Cytometry Immunoglobulin E/metabolism Lymphocytes/immunology Macromolecular Substances Major Histocompatibility Complex Mice Mice, Inbred C3H Mice, Inbred Strains Polymerase Chain Reaction Receptors, Antigen, T-Cell/genetics Receptors, Fc/genetics Receptors, IgE Spleen/immunology T-Lymphocytes/immunology
Chemicals
Antigens, Differentiation, B-Lymphocyte Antigens, Differentiation, T-Lymphocyte CD3 Complex Macromolecular Substances Receptors, Antigen, T-Cell Receptors, Fc Receptors, IgE Immunoglobulin E
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Koyasu S
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
D'Adamio L
Arulanandam A R
Abraham S
Clayton L K
Reinherz E L
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.175.1.203
Published
1992-01-01
Pages
203-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2119082
Subset
IM
Grants
NIAID NIH HHS · AI-19807 · United States
NIAID NIH HHS · AI-21226 · United States
NIAID NIH HHS · AI-31269 · United States
External Links
PubMed source DOI: 10.1084/jem.175.1.203 PMC Full Text View journal details
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