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PMID: 1531384 Published · ppublish English Journal Article

A new method for the isolation of recombinant baculovirus.

Nucleic acids research ·Vol. 20 ·No. 1 ·1992-01-11 ·Pages 97-104

Patel G, Nasmyth K, Jones N

Abstract

An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectable marker. The viral DNA maintained its ability to replicate in insect cells. An efficient and rapid selection system was set up, to isolate viral recombinants in yeast; DNA from selected yeast colonies was transfected into insect cells to obtain recombinant virus. We demonstrate the utility of this system by isolating recombinant viruses that express two different members of the CREB/ATF family of transcription factors.

Related Genes
CREB1 CREB2 SUP4-o URA3
MeSH Terms
Animals Baculoviridae/genetics,isolation & purification Base Sequence Cell Line Cyclic AMP Response Element-Binding Protein DNA, Recombinant/genetics DNA, Viral/genetics DNA-Binding Proteins/genetics Genetic Vectors/genetics Humans Insecta/genetics Molecular Sequence Data Plasmids/genetics Saccharomyces cerevisiae/genetics Transcription Factors/genetics
Chemicals
Cyclic AMP Response Element-Binding Protein DNA, Recombinant DNA, Viral DNA-Binding Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Patel G
Gene Regulation Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
Nasmyth K
Jones N
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.1.97
Published
1992-01-11
Pages
97-104
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310331
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.1.97 PMC Full Text View journal details
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