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PMID: 2147221 Published · ppublish English Journal Article

The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6192-203

Hurst HC, Masson N, Jones NC, Lee KA

Abstract

Promoter elements containing the sequence motif CGTCA are important for a variety of inducible responses at the transcriptional level. Multiple cellular factors specifically bind to these elements and are encoded by a multigene family. Among these factors, polypeptides termed activating transcription factor 43 (ATF-43) and ATF-47 have been purified from HeLa cells and a factor referred to as cyclic AMP response element-binding protein (CREB) has been isolated from PC12 cells and rat brain. We demonstrated that CREB and ATF-47 are identical and that CREB and ATF-43 form protein-protein complexes. We also found that the cis requirements for stable DNA binding by ATF-43 and CREB are different. Using antibodies to ATF-43 we have identified a group of polypeptides (ATF-43) in the size range from 40 to 43 kDa. ATF-43 polypeptides are related by their reactivity with anti-ATF-43, DNA-binding specificity, complex formation with CREB, heat stability, and phosphorylation by protein kinase A. Certain cell types vary in their ATF-43 complement, suggesting that CREB activity is modulated in a cell-type-specific manner through interaction with ATF-43. ATF-43 polypeptides do not appear simply to correspond to the gene products of the ATF multigene family, suggesting that the size of the ATF family at the protein level is even larger than predicted from cDNA-cloning studies.

MeSH Terms
Activating Transcription Factors Antibodies Base Sequence Blood Proteins/genetics,isolation & purification,metabolism Cell Line Cell Nucleus/metabolism Chromatography, Affinity Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins/genetics,metabolism HeLa Cells/metabolism Humans Immunoblotting Molecular Sequence Data Oligonucleotide Probes Phosphorylation Promoter Regions, Genetic Protein Binding Protein Biosynthesis Protein Kinases/metabolism Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic
Chemicals
Activating Transcription Factors Antibodies Blood Proteins Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins Oligonucleotide Probes Transcription Factors Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hurst H C
Gene Transcription Group, Imperial Cancer Research Fund, Hammersmith Hospital, London.
Masson N
Jones N C
Lee K A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6192-6203.1990
Published
1990-12-00
Pages
6192-203
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362894
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.12.6192-6203.1990 PMC Full Text View journal details
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