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PMID: 2542018 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bovine leukemia virus trans-activator p38tax activates heterologous promoters with a common sequence known as a cAMP-responsive element or the binding site of a cellular transcription factor ATF.

The EMBO journal ·Vol. 8 ·No. 2 ·1989-02-00 ·Pages 497-503

Katoh I, Yoshinaka Y, Ikawa Y

Abstract

The genome of bovine leukemia virus (BLV) encodes a transcriptional trans-activator p38tax (also referred to as pXBL-I) which amplifies the virus gene expression driven by its long terminal repeat (LTR). It was proposed that activation of cellular gene expression by p38tax might be involved in the mechanism of B-cell transformation caused in vivo by BLV infection. Here, we report that the U3 region of BLV LTR contains multiple regulatory elements responsive to p38tax. A core element composing the p38tax-inducible U3 structure is suggested to be a heptanucleotide motif of 5'TGACGTCA3', the consensus sequence proposed for a cAMP-responsive element (CRE) and for the binding sites of a cellular transcription factor (ATF). Adenovirus-5 E3 and E4, c-fos and somatostatin regulatory regions containing CRE/ATF-element exhibited responsiveness to p38tax in a chloramphenicol acetyltransferase transient expression assay. These suggest that in BLV-infected cells, cellular gene expression might be induced abnormally by the virus trans-activator through ATF or ATF-like factors.

MeSH Terms
Activating Transcription Factors Animals Base Sequence Binding Sites Blood Proteins/genetics,metabolism Chloramphenicol O-Acetyltransferase/genetics Cyclic AMP/genetics,metabolism DNA, Viral/genetics Enhancer Elements, Genetic Gene Expression Regulation Gene Products, tax Leukemia Virus, Bovine/genetics Promoter Regions, Genetic/drug effects Proto-Oncogenes Retroviridae/genetics Retroviridae Proteins/genetics,pharmacology Retroviridae Proteins, Oncogenic Transcription Factors/genetics,metabolism
Chemicals
Activating Transcription Factors Blood Proteins DNA, Viral Gene Products, tax Retroviridae Proteins Retroviridae Proteins, Oncogenic Transcription Factors transactivator protein p38(tax) Cyclic AMP Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Katoh I
Tsukuba Life Science Center, Institute of Chemical and Physical Research, Ibaraki, Japan.
Yoshinaka Y
Ikawa Y
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-02-00
Pages
497-503
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400832
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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