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PMID: 1532257 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 6 ·1992-03-15 ·Pages 2489-93

Hatada EN, Nieters A, Wulczyn FG, Naumann M, Meyer R, Nucifora G, McKeithan TW, Scheidereit C

Abstract

The inducible pleiotropic transcription factor NF-kappa B is composed of two subunits, p50 and p65. The p50 subunit is encoded on the N-terminal half of a 105-kDa open reading frame and contains a rel-like domain. To date, no function has been described for the C-terminal portion. We show here that the C-terminal half of p105, when expressed as a separate molecule, binds to p50 and can rapidly disrupt protein-DNA complexes of p50 or native NF-kappa B. Deletion analysis of this precursor-derived inhibitor activity indicated a domain containing ankyrin-like repeats as necessary for inhibition. The protooncogene bcl-3, which contains seven ankyrin repeats, can equally inhibit p50 DNA binding. These observations identify bcl-3 as an inhibitor of NF-kappa B and strongly suggest that the ankyrin repeats in these factors are involved in protein-protein interactions with the rel-like domain of p50. Comparison with other ankyrin repeat-containing proteins suggests that a subclass of these proteins acts as regulators of rel-like transcription factors.

Related Genes

bcl-3

MeSH Terms

Amino Acid Sequence Ankyrins B-Cell Lymphoma 3 Protein Blood Proteins/genetics,metabolism Cell Line DNA-Binding Proteins/genetics Gene Expression Regulation, Neoplastic HeLa Cells Humans Macromolecular Substances Membrane Proteins/genetics,metabolism Molecular Sequence Data NF-kappa B/genetics,metabolism NF-kappa B p50 Subunit Protein Precursors/genetics,metabolism Proto-Oncogene Proteins/genetics Proto-Oncogenes Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription Factors

Chemicals

Ankyrins B-Cell Lymphoma 3 Protein BCL3 protein, human Blood Proteins DNA-Binding Proteins Macromolecular Substances Membrane Proteins NF-kappa B NF-kappa B p50 Subunit Protein Precursors Proto-Oncogene Proteins Transcription Factors

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Hatada E N

Max-Planck-Institut fuer Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin-Dahlem, Federal Republic of Germany.

Nieters A

Wulczyn F G

Naumann M

Meyer R

Nucifora G

McKeithan T W

Scheidereit C

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.89.6.2489

Published

1992-03-15

Pages

2489-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC48684

Subset

External Links

PubMed source DOI: 10.1073/pnas.89.6.2489 PMC Full Text View journal details

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The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.

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