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PMID: 1532387 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli.

Journal of bacteriology ·Vol. 174 ·No. 7 ·1992-04-00 ·Pages 2145-51

Polarek JW, Williams G, Epstein W

Abstract

The expression of the Kdp system for K+ uptake in Escherichia coli requires the products of two genes, kdpD and kdpE. These genes constitute an operon adjacent to the kdpABC operon that encodes the three membrane protein subunits of Kdp. Both operons are transcribed in the same direction and overlap; the kdpDE promoter is in kdpC, the last gene of the kdpABC operon. Transcription of the kdpDE operon is at a low level when Kdp is not expressed; transcription increases about 10-fold when kdpABC is turned on, indicating significant read-through of the kdpDE operon by transcripts beginning at the promoter of kdpABC operon. The proximal region of the kdpD gene is the site of most mutations that lead to constitutive expression of the kdpABC operon.

Related Genes
kdpD kdpE
MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Biological Transport, Active Cation Transport Proteins Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Molecular Sequence Data Operon Potassium/metabolism Promoter Regions, Genetic Restriction Mapping Transcription, Genetic
Chemicals
Bacterial Proteins Cation Transport Proteins DNA, Bacterial Adenosine Triphosphatases potassium transporting ATPase Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Polarek J W
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Williams G
Epstein W
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.7.2145-2151.1992
Published
1992-04-00
Pages
2145-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205832
Subset
IM
Grants
NIGMS NIH HHS · GM07197 · United States
NIGMS NIH HHS · GM22323 · United States
External Links
PubMed source DOI: 10.1128/jb.174.7.2145-2151.1992 PMC Full Text View journal details
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