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PMID: 15337848 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recruitment of the Puf3 protein to its mRNA target for regulation of mRNA decay in yeast.

RNA (New York, N.Y.) ·Vol. 10 ·No. 10 ·2004-10-00 ·Pages 1625-36

Jackson JS, Houshmandi SS, Lopez Leban F, Olivas WM

Abstract

The Puf family of RNA-binding proteins regulates mRNA translation and decay via interactions with 3' untranslated regions (3' UTRs) of target mRNAs. In yeast, Puf3p binds the 3' UTR of COX17 mRNA and promotes rapid deadenylation and decay. We have investigated the sequences required for Puf3p recruitment to this 3' UTR and have identified two separate binding sites. These sites are specific for Puf3p, as they cannot bind another Puf protein, Puf5p. Both sites use a conserved UGUANAUA sequence, whereas one site contains additional sequences that enhance binding affinity. In vivo, presence of either site partially stimulates COX17 mRNA decay, but full decay regulation requires the presence of both sites. No other sequences outside the 3' UTR are required to mediate this decay regulation. The Puf repeat domain of Puf3p is sufficient not only for in vitro binding to the 3' UTR, but also in vivo stimulation of COX17 mRNA decay. These experiments indicate that the essential residues involved in mRNA decay regulation are wholly contained within this RNA-binding domain.

MeSH Terms
3' Untranslated Regions Base Sequence Binding Sites/genetics Cation Transport Proteins/genetics,metabolism Copper Transport Proteins Molecular Chaperones Molecular Sequence Data RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
3' Untranslated Regions COX17 protein, S cerevisiae Cation Transport Proteins Copper Transport Proteins Molecular Chaperones RNA, Fungal RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jackson John S
Department of Biology, University of Missouri-St. Louis, One University Boulevard, St. Louis, MO 63121-4499, USA.
Houshmandi S Sean
Lopez Leban Florencia
Olivas Wendy M
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.7270204
Published
2004-10-00
Epub
2004-00-30
Pages
1625-36
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370648
Subset
IM
Grants
NIGMS NIH HHS · R01 GM063759 · United States
NIGMS NIH HHS · GM63759 · United States
External Links
PubMed source DOI: 10.1261/rna.7270204 PMC Full Text View journal details
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