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PMID: 1539985 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50.

Applied and environmental microbiology ·Vol. 58 ·No. 1 ·1992-01-00 ·Pages 399-402

Layton AC, Sanseverino J, Wallace W, Corcoran C, Sayler GS

Abstract

The biodegradation of 4-chlorobiphenyl usually proceeds through the intermediate 4-chlorobenzoate. Few bacterial strains can degrade 4-chlorobiphenyl to 4-chlorobenzoate and 4-chlorobenzoate to CO2. This study demonstrates that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

MeSH Terms
Alcaligenes/metabolism Biodegradation, Environmental Chlorides/metabolism Chlorobenzoates/metabolism Halogens/metabolism Plasmids Restriction Mapping
Chemicals
Chlorides Chlorobenzoates Halogens 4-chlorobenzoic acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Layton A C
Department of Microbiology, University of Tennessee, Knoxville 37932.
Sanseverino J
Wallace W
Corcoran C
Sayler G S
References (16)
16 references, click to expand
  1. Comparative effects of Aroclor 1254 (polychlorinated biphenyls) and phenanthrene on glucose uptake by freshwater microbial populations.
    Appl Environ Microbiol. 1979 May;37(5):878-85 PMID: 114110
  2. Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp.
    Appl Environ Microbiol. 1984 Nov;48(5):1020-5 PMID: 16346660
  3. Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4'-dichlorobiphenyl.
    Appl Environ Microbiol. 1989 Apr;55(4):887-92 PMID: 2499257
  4. Plasmid-mediated mineralization of 4-chlorobiphenyl.
    J Bacteriol. 1985 Sep;163(3):882-9 PMID: 2993249
  5. A novel method for rapid isolation of plasmid DNA.
    Biotechniques. 1988 Mar;6(3):238-42 PMID: 3078517
  6. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  7. Involvement of plasmids in total degradation of chlorinated biphenyls.
    Appl Environ Microbiol. 1982 Sep;44(3):619-26 PMID: 6814360
  8. Novel enzymic hydrolytic dehalogenation of a chlorinated aromatic.
    Science. 1991 Jul 12;253(5016):182-5 PMID: 1853203
  9. Chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria.
    Appl Environ Microbiol. 1990 Jul;56(7):2036-45 PMID: 2117875
  10. Comparative genetic organization of incompatibility group P degradative plasmids.
    J Bacteriol. 1990 Dec;172(12):6818-25 PMID: 2254257
  11. Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.
    Appl Environ Microbiol. 1989 May;55(5):1286-8 PMID: 2757383
  12. Microbial degradation of haloaromatics.
    Annu Rev Microbiol. 1988;42:263-87 PMID: 3059995
  13. Reductive dechlorination of 2,4-dichlorobenzoate to 4-chlorobenzoate and hydrolytic dehalogenation of 4-chloro-, 4-bromo-, and 4-iodobenzoate by Alcaligenes denitrificans NTB-1.
    Appl Environ Microbiol. 1987 Apr;53(4):810-5 PMID: 3579283
  14. Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate.
    J Bacteriol. 1986 Oct;168(1):81-5 PMID: 3759912
  15. Microbial biodegradation of 4-chlorobiphenyl, a model compound of chlorinated biphenyls.
    Appl Environ Microbiol. 1984 May;47(5):947-51 PMID: 6742836
  16. Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components.
    Appl Environ Microbiol. 1991 Jan;57(1):324-6 PMID: 2036019
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.58.1.399-402.1992
Published
1992-01-00
Pages
399-402
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC195221
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.58.1.399-402.1992 PMC Full Text View journal details
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