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PMID: 2757383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.

Applied and environmental microbiology ·Vol. 55 ·No. 5 ·1989-05-00 ·Pages 1286-8

Hooper SW, Dockendorff TC, Sayler GS

Abstract

The plasmid pSS50 is a 53-kilobase self-transmissible plasmid of broad host range that has been isolated from several Alcaligenes and Acinetobacter species. This plasmid has previously been shown to mediate the mineralization of 4-chlorobiphenyl to carbon dioxide and water. Physical characterization of this plasmid by restriction analysis indicates that most hexanucleotide cleavage sites are clustered in a 5-kilobase region, leaving large regions without restriction sites. The paucity of restriction sites is not due to DNA methylation.

MeSH Terms

Acinetobacter/genetics,metabolism Alcaligenes/genetics,metabolism Biodegradation, Environmental Biphenyl Compounds/metabolism DNA, Bacterial/analysis Plasmids Restriction Mapping

Chemicals

Biphenyl Compounds DNA, Bacterial 4-chlorobiphenyl

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hooper S W

Department of Microbiology, University of Tennessee, Knoxville 37932-2567.

Dockendorff T C

Sayler G S

References (13)

13 references, click to expand

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Recognition sequences of restriction endonucleases and methylases--a review.
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Plasmid-mediated mineralization of 4-chlorobiphenyl.
J Bacteriol. 1985 Sep;163(3):882-9 PMID: 2993249
Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes.
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Novel biotransformations of 4-chlorobiphenyl by a Pseudomonas sp.
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.55.5.1286-1288.1989

Published

1989-05-00

Pages

1286-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC184292

Subset

Grants

PHS HHS · T-32-A107123-06 · United States

External Links

PubMed source DOI: 10.1128/aem.55.5.1286-1288.1989 PMC Full Text View journal details

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Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.

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