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PMID: 1544570 Published · ppublish English Journal Article

Reporter constructs with low background activity utilizing the cat gene.

Gene ·Vol. 110 ·No. 1 ·1992-01-02 ·Pages 129-30

Boshart M, Klüppel M, Schmidt A, Schütz G, Luckow B

Abstract

Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

Related Genes
cat
MeSH Terms
Animals Base Sequence Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Genes, Regulator Genetic Vectors Molecular Sequence Data Plasmids Promoter Regions, Genetic Rats Tumor Cells, Cultured
Chemicals
Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Boshart M
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Klüppel M
Schmidt A
Schütz G
Luckow B
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(92)90456-y
Published
1992-01-02
Pages
129-30
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Databases
GENBANK
M80483, M80484, S39996, S40001, S40008, S40009, S40010, S46736, S46737, X72580
External Links
PubMed source DOI: 10.1016/0378-1119(92)90456-y View journal details
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