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PMID: 15449020 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Non-coordinate expression of peroxisome biogenesis, beta-oxidation and glyoxylate cycle genes in mature Arabidopsis plants.

Plant cell reports ·Vol. 23 ·No. 9 ·2005-02-00 ·Pages 647-53

Charlton WL, Johnson B, Graham IA, Baker A

Abstract

The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in young seedlings and the root tips of older plants. PEX1 and 3-ketoacyl thiolase (PED1) were expressed in roots, mature leaves, stems and flowers. However, only thiolase was up-regulated by starvation. Immunoblotting confirmed that neither malate synthase nor the other unique glyoxylate cycle enzyme isocitrate lyase are expressed in senescent leaves. These results indicate that, in contrast to cucumber, pumpkin and barley, the glyoxylate cycle does not play a role in the recycling of carbon from the turnover of membrane lipids during senescence and starvation in Arabidopsis.

MeSH Terms
Arabidopsis/enzymology,genetics Arabidopsis Proteins/genetics,metabolism Cloning, Molecular Cucumis sativus/enzymology,genetics Cucurbita/enzymology,genetics Gene Expression Regulation, Plant/genetics,physiology Genes, Reporter/genetics Glyoxylates/metabolism Hordeum/enzymology,genetics Malate Synthase/genetics,metabolism Oxidation-Reduction Peroxisomes/enzymology,genetics Plant Leaves/enzymology,genetics Plant Roots/enzymology,genetics Promoter Regions, Genetic
Chemicals
Arabidopsis Proteins Glyoxylates Malate Synthase glyoxylic acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charlton Wayne L
Centre for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Johnson Barbara
Graham Ian A
Baker Alison
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Article Info
Journal
Plant cell reports
Abbr.
Plant Cell Rep
ISSN
0721-7714
DOI
10.1007/s00299-004-0879-7
Published
2005-02-00
Epub
2004-00-22
Pages
647-53
Language
English
Region
Germany
NLM ID
9880970
Subset
IM
External Links
PubMed source DOI: 10.1007/s00299-004-0879-7 View journal details
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