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PMID: 1545784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies.

Molecular biology reports ·Vol. 16 ·No. 1 ·1992-02-00 ·Pages 49-59

Verheijen R, de Jong BA, Oberyé EH, van Venrooij WJ

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of anticentromere autoantibodies in sera of patients with suspected or manifest rheumatic diseases. The antigen source used in this assay consists of the recombinant protein of glutathione S-transferase (GST) fused to the last 60 C-terminal amino acid residues of the major centromere protein CENP-B. Although this CENP-B segment is only a small part of the complete polypeptide, we show that it constitutes an important autoimmune antigenic domain which is recognized by all patient sera in which ACA can be detected using the immunoblotting technique with a HeLa S3 nuclear protein extract as antigen source.

MeSH Terms

Amino Acid Sequence Autoantibodies/blood,immunology Autoantigens/genetics,immunology Centromere/immunology Centromere Protein B Chromosomal Proteins, Non-Histone/genetics,immunology DNA/genetics DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Escherichia coli/genetics,metabolism Glutathione Transferase/genetics Humans Molecular Sequence Data Recombinant Fusion Proteins/genetics Rheumatic Diseases/genetics,immunology

Chemicals

Autoantibodies Autoantigens CENPB protein, human Centromere Protein B Chromosomal Proteins, Non-Histone DNA-Binding Proteins Recombinant Fusion Proteins DNA Glutathione Transferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Verheijen R

Department of Biochemistry, University of Nijmegen, The Netherlands.

de Jong B A

Oberyé E H

van Venrooij W J

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Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1007/BF00788753

Published

1992-02-00

Pages

49-59

Language

English

Region

Netherlands

NLM ID

0403234

Subset

External Links

PubMed source DOI: 10.1007/BF00788753 View journal details

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Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies.

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