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PMID: 15466620 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Basophils initiate IL-4 production during a memory T-dependent response.

The Journal of experimental medicine ·Vol. 200 ·No. 7 ·2004-10-04 ·Pages 857-70

Khodoun MV, Orekhova T, Potter C, Morris S, Finkelman FD

Abstract

Experiments were performed to characterize and identify the cellular sources of the secondary interleukin (IL)-4 response to a T cell-dependent antigen. Mice were primed by immunization with goat anti-mouse immunoglobulin (Ig)D antibody (GaMD), which stimulates naive CD4+ T cells to secrete IL-4 in 3-4 d. When challenged with goat serum 14 d after immunization, GaMD-primed mice generated an IL-4 response that exceeded the primary response by approximately 100-fold, started in <2 h, and lasted for 4 d. Studies with 4get mice, in which cells with an accessible Il4 gene express a green fluorescent protein (GFP), revealed CD4+ memory T cells, natural killer T cells, basophils, mast cells, and eosinophils as possible rapid producers of IL-4. GFP(+)CD4+ T cells and basophils expanded more in the spleen than the other cell types during the primary response to GaMD. Quantitation of in vivo IL-4 production by the in vivo cytokine capture assay after individual cell types were selectively stimulated or deleted demonstrated that basophils and memory CD4+ T cells account for most of the secondary IL-4 response, with basophils initiating that response through IgE/FcepsilonRI-mediated signaling but secreting IL-4 for <4 h and memory T cells secreting IL-4 within 4 h and continuing to secrete this cytokine for 4 d.

MeSH Terms
Animals Basophils/immunology,metabolism Chymases Cytokines/immunology Flow Cytometry Fluorescent Antibody Technique Green Fluorescent Proteins/metabolism Immunization Immunologic Memory Interleukin-4/biosynthesis,metabolism Mice Mice, Inbred BALB C Serine Endopeptidases/blood Spleen/immunology T-Lymphocytes/immunology,metabolism Time Factors
Chemicals
Cytokines Green Fluorescent Proteins Interleukin-4 Serine Endopeptidases Chymases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Khodoun Marat V
Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Orekhova Tatyana
Potter Crystal
Morris Suzanne
Finkelman Fred D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20040598
Published
2004-10-04
Pages
857-70
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2213291
Subset
IM
Grants
NIAID NIH HHS · R01 AI44971 · United States
NHLBI NIH HHS · P01 HL076383 · United States
NIAID NIH HHS · R01 AI45766 · United States
NIAID NIH HHS · R01 AI055848 · United States
NIAID NIH HHS · R01 AI045766 · United States
NHLBI NIH HHS · HL0763833 · United States
NIAID NIH HHS · R01 AI55848 · United States
External Links
PubMed source DOI: 10.1084/jem.20040598 PMC Full Text View journal details
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