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PMID: 15470259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of flagellar assembly by glycogen synthase kinase 3 in Chlamydomonas reinhardtii.

Eukaryotic cell ·Vol. 3 ·No. 5 ·2004-10-00 ·Pages 1307-19

Wilson NF, Lefebvre PA

Abstract

Chlamydomonas reinhardtii controls flagellar assembly such that flagella are of an equal and predetermined length. Previous studies demonstrated that lithium, an inhibitor of glycogen synthase kinase 3 (GSK3), induced flagellar elongation, suggesting that a lithium-sensitive signal transduction pathway regulated flagellar length (S. Nakamura, H. Takino, and M. K. Kojima, Cell Struct. Funct. 12:369-374, 1987). Here, we demonstrate that lithium treatment depletes the pool of flagellar proteins from the cell body and that the heterotrimeric kinesin Fla10p accumulates in flagella. We identify GSK3 in Chlamydomonas and demonstrate that its kinase activity is inhibited by lithium in vitro. The tyrosine-phosphorylated, active form of GSK3 was enriched in flagella and GSK3 associated with the axoneme in a phosphorylation-dependent manner. The level of active GSK3 correlated with flagellar length; early during flagellar regeneration, active GSK3 increased over basal levels. This increase in active GSK3 was rapidly lost within 30 min of regeneration as the level of active GSK3 decreased relative to the predeflagellation level. Taken together, these results suggest a possible role for GSK3 in regulating the assembly and length of flagella.

MeSH Terms
Algal Proteins Amino Acid Sequence Animals Base Sequence Chlamydomonas reinhardtii/enzymology,genetics,growth & development Cloning, Molecular DNA, Protozoan/genetics Enzyme Inhibitors/pharmacology Flagella/drug effects,enzymology,physiology Genes, Protozoan Glycogen Synthase Kinase 3/antagonists & inhibitors,genetics,metabolism Lithium Chloride/pharmacology Microtubule-Associated Proteins/genetics,metabolism Molecular Sequence Data Phenotype Phosphorylation Protozoan Proteins/genetics,metabolism Sequence Homology, Amino Acid Tyrosine/metabolism
Chemicals
Algal Proteins DNA, Protozoan Enzyme Inhibitors KHP1 protein, Chlamydomonas Microtubule-Associated Proteins Protozoan Proteins Tyrosine Glycogen Synthase Kinase 3 Lithium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson Nedra F
Department of Plant Biology, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108, USA. [email protected].
Lefebvre Paul A
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.3.5.1307-1319.2004
Email
[email protected]
Published
2004-10-00
Pages
1307-19
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC522593
Subset
IM
Grants
NIGMS NIH HHS · F32 GM020149 · United States
NIGMS NIH HHS · R01 GM034437 · United States
NIGMS NIH HHS · 5F32-GM20149 · United States
NIGMS NIH HHS · GM34437 · United States
External Links
PubMed source DOI: 10.1128/EC.3.5.1307-1319.2004 PMC Full Text View journal details
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