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PMID: 15472079 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A mechanism related to the yeast transcriptional regulator Paf1c is required for expression of the Arabidopsis FLC/MAF MADS box gene family.

The Plant cell ·Vol. 16 ·No. 11 ·2004-11-00 ·Pages 2940-53

Oh S, Zhang H, Ludwig P, van Nocker S

Abstract

The Arabidopsis thaliana VERNALIZATION INDEPENDENCE (VIP) gene class has multiple functions in development, including repression of flowering through activation of the MADSbox gene FLC. Epigenetic silencing of FLC plays a substantial role in the promotion of flowering through cold (vernalization). To better understand how VIP genes influence development, we undertook a genetic and molecular study of the previously uncharacterized VIP5 and VIP6 genes. We found that loss of function of these genes also resulted in downregulation of other members of the FLC/MAF gene family, including the photoperiodic pathway regulator MAF1/FLM. We cloned VIP5 and VIP6 through mapping and transcriptional profiling. Both proteins are closely related to distinct components of budding yeast Paf1C, a transcription factor that assists in establishment and maintenance of transcription-promotive chromatin modifications such as ubiquitination of H2B by Bre1/Rad6 and methylation of histone H3 lysine-4 by the trithorax-related histone methylase Set1. Genetic analysis and coimmunoprecipitation experiments suggest that VIP5 and VIP6 function in the same mechanism as the previously described VIP3 and VIP4. Our findings suggest that an evolutionarily conserved transcriptional mechanism plays an essential role in the maintenance of gene expression in higher eukaryotes and has a central function in flowering.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,isolation & purification,metabolism Down-Regulation/genetics Flowers/genetics,metabolism Gene Expression Regulation, Plant/genetics Gene Silencing/physiology Genes, Regulator/genetics Histones/metabolism MADS Domain Proteins/genetics,metabolism Matrix Attachment Region Binding Proteins/genetics,metabolism Methylation Nuclear Proteins/genetics Plant Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics Transcription Factors/genetics,isolation & purification,metabolism
Chemicals
Arabidopsis Proteins FLF protein, Arabidopsis Histones MADS Domain Proteins Matrix Attachment Region Binding Proteins Nuclear Proteins PAF1 protein, S cerevisiae Plant Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oh Sookyung
Plant Breeding and Genetics, Michigan State University, East Lansing, Michigan 48824, USA.
Zhang Hua
Ludwig Philip
van Nocker Steven
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.026062
Published
2004-11-00
Epub
2004-00-07
Pages
2940-53
Language
English
Region
England
NLM ID
9208688
PMCID
PMC527190
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.104.026062 PMC Full Text View journal details
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