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PMID: 1549125 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical beta-cell nuclear complexes.

Molecular and cellular biology ·Vol. 12 ·No. 4 ·1992-04-00 ·Pages 1777-88

German MS, Moss LG, Wang J, Rutter WJ

Abstract

The pancreatic beta cell makes several unique gene products, including insulin, islet amyloid polypeptide (IAPP), and beta-cell-specific glucokinase (beta GK). The functions of isolated portions of the insulin, IAPP, and beta GK promoters were studied by using transient expression and DNA binding assays. A short portion (-247 to -197 bp) of the rat insulin I gene, the FF minienhancer, contains three interacting transcriptional regulatory elements. The FF minienhancer binds at least two nuclear complexes with limited tissue distribution. Sequences similar to that of the FF minienhancer are present in the 5' flanking DNA of the human IAPP and rat beta GK genes and also the rat insulin II and mouse insulin I and II genes. Similar minienhancer constructs from the insulin and IAPP genes function as cell-specific transcriptional regulatory elements and compete for binding of the same nuclear factors, while the beta GK construct competes for protein binding but functions poorly as a minienhancer. These observations suggest that the patterns of expression of the beta-cell-specific genes result in part from sharing the same transcriptional regulators.

MeSH Terms
Amyloid/genetics Animals Base Sequence Binding, Competitive Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase DNA Mutational Analysis DNA-Binding Proteins/metabolism Enhancer Elements, Genetic/genetics Gene Expression Regulation Insulin/genetics Islet Amyloid Polypeptide Islets of Langerhans/metabolism Molecular Sequence Data Promoter Regions, Genetic/genetics Rats Recombinant Fusion Proteins Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Amyloid DNA-Binding Proteins Insulin Islet Amyloid Polypeptide Recombinant Fusion Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
German M S
Hormone Research Institute, University of California, San Francisco 94143.
Moss L G
Wang J
Rutter W J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.4.1777-1788.1992
Published
1992-04-00
Pages
1777-88
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369621
Subset
IM
Grants
NIDDK NIH HHS · R01 DK021344 · United States
NIDDK NIH HHS · DK-21344 · United States
NIDDK NIH HHS · DK-43129 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.4.1777-1788.1992 PMC Full Text View journal details
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