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PMID: 3043185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Systematic binding analysis of the insulin gene transcription control region: insulin and immunoglobulin enhancers utilize similar transactivators.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2620-7

Moss LG, Moss JB, Rutter WJ

Abstract

The 5' regulatory region (-345 to +1) of the rat insulin I gene (Ins-I) was examined for binding to cellular factors with short oligodeoxynucleotide probes. Over 40 binding species were detected. The binding profiles were specific for each cell type studied. We characterized the factors binding two elements crucial for enhancer activity (the Nir and Far boxes) which bear sequence similarity to the microE1, microE2, and microE3 elements of the immunoglobulin heavy-chain enhancer. The Nir box binds three cellular factors that display preferential affinities for microE1, microE2, or microE3, and the Far box binds two factors related to microE2 or microE3. The insulin gene enhancer was mutated at the Nir box element to reflect the sequences of microE1, microE2, or microE3. Ins-microE2 was fully active, Ins-microE3 was partially active, and Ins-microE1 was inactive. Thus, factors similar or identical to nuclear factor NF-microE1, NF-microE2, or NF-microE3 may play a role in the activity of the insulin gene enhancer.

MeSH Terms
Animals DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Gene Expression Regulation Genes, Immunoglobulin Insulin/genetics Mutation Rats Transcription, Genetic Transfection
Chemicals
DNA-Binding Proteins Insulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moss L G
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Moss J B
Rutter W J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2620-2627.1988
Published
1988-06-00
Pages
2620-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363464
Subset
IM
Grants
NIADDK NIH HHS · AM21344 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.6.2620-2627.1988 PMC Full Text View journal details
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