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PMID: 6961401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 23 ·1982-12-00 ·Pages 7132-6

Hen R, Sassone-Corsi P, Corden J, Gaub MP, Chambon P

Abstract

We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA, Viral/genetics Gene Expression Regulation HeLa Cells Operon Transcription, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hen R
Sassone-Corsi P
Corden J
Gaub M P
Chambon P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.23.7132
Published
1982-12-00
Pages
7132-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347292
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.23.7132 PMC Full Text View journal details
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