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PMID: 6985482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region.

Nucleic acids research ·Vol. 9 ·No. 16 ·1981-08-25 ·Pages 4023-42

Hérissé J, Rigolet M, de Dinechin SD, Galibert F

Abstract

The entire nucleotide sequence between coordinates 89.5 and 100% of the Ad 2 DNA genome has been determined using the Maxam and Gilbert method. This sequence of 3766 bp contains information relative to the carboxylic end of the fiber protein and to the entire E4 region. The position within the nucleotide sequence of various open reading frames and of several consensus splicing sequences was correlated with the location by EM and Sl digestion of the E4 mRNA. This correlation allows to suggest an additional splicing event in the maturation process of i or f mRNA and to deduce the structure of most E4 mRNA. The aminoacid sequences of the corresponding proteins are deduced allowing the location of several glycosylation sites. The presence of several open reading frames with a substantial coding capacity permits to postulate on the existence of additional genes located at the 3' end of the fiber gene and the 3' end of the E4 region. The existence of these putative additional genes might explain that termination of transcription is several hundred nucleotides beyond the main known poly A addition sites of the L5 and E4 regions.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Chromosome Mapping DNA, Viral/genetics Genes, Viral Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
DNA, Viral RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hérissé J
Rigolet M
de Dinechin S D
Galibert F
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.16.4023
Published
1981-08-25
Pages
4023-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327412
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1093/nar/9.16.4023 PMC Full Text View journal details
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