Home LiteratureArticle Details
PMID: 1549474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A superfamily of Drosophila satellite related (SR) DNA repeats restricted to the X chromosome euchromatin.

Nucleic acids research ·Vol. 20 ·No. 5 ·1992-03-11 ·Pages 1113-6

DiBartolomeis SM, Tartof KD, Jackson FR

Abstract

The 1.688 g/cm3 class of Drosophila satellite DNA is predominantly localized to the centromeric heterochromatin of the X chromosome. We report here the existence of 1.688 satellite related (SR) DNA arrays present at numerous locations throughout the euchromatic portion of the X. Unlike their heterochromatic counterparts, euchromatic SRs consist of a small number of repeating units (usually 2-4), each of which is 63-81% identical to the 359-bp monomer of the 1.688 satellite. Although it appears that SR DNA arrays are not transcribed, in at least two cases, they are located adjacent to transcriptionally active genes. SR sequences also have significant similarity to a previously described Drosophila middle repeat found almost exclusively in the X euchromatin. It seems likely that these X linked sequences are required for sex chromosome specific functions.

MeSH Terms
Animals Chromatin DNA, Satellite/genetics Drosophila melanogaster/genetics Euchromatin Molecular Sequence Data Nucleic Acid Conformation Repetitive Sequences, Nucleic Acid/genetics X Chromosome
Chemicals
Chromatin DNA, Satellite Euchromatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DiBartolomeis S M
Neurobiology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Tartof K D
Jackson F R
References (15)
15 references, click to expand
  1. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  2. The role of X-chromosome inactivation during spermatogenesis (Drosophila-allocycly-chromosome evolution-male sterility-dosage compensation).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):182-6 PMID: 4621547
  3. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  4. Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7152-6 PMID: 6818540
  5. The genomics of long tandem arrays of satellite DNA in the human genome.
    Genome. 1989;31(2):737-44 PMID: 2698839
  6. Gene dosage compensation in Drosophila melanogaster.
    Adv Genet. 1987;24:371-429 PMID: 3124533
  7. A structural basis for variegating position effects.
    Cell. 1984 Jul;37(3):869-78 PMID: 6086148
  8. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  9. Sequence and sequence variation within the 1.688 g/cm3 satellite DNA of Drosophila melanogaster.
    J Mol Biol. 1979 Dec 5;135(2):465-81 PMID: 231676
  10. Different regions of a complex statellite DNA vary in size and sequence of the repeating unit.
    J Mol Biol. 1979 Dec 5;135(2):483-500 PMID: 231677
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila.
    Cell. 1991 Sep 6;66(5):935-47 PMID: 1653648
  13. Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.
    Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6795-9 PMID: 1907376
  14. Cloning and characterization of a dispersed, multicopy, X chromosome sequence in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2843-7 PMID: 3106978
  15. A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
    Cell. 1984 Jul;37(3):889-901 PMID: 6540146
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.5.1113
Published
1992-03-11
Pages
1113-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312099
Subset
IM
Grants
NCI NIH HHS · CA-06927 · United States
NINDS NIH HHS · NS30386 · United States
NCRR NIH HHS · RR-05539 · United States
Databases
GENBANK
X62937, X62938, X62939
External Links
PubMed source DOI: 10.1093/nar/20.5.1113 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2