Improved primer design for PCR-based, site-directed mutagenesis.

Nucleic acids research ·Vol. 20 ·No. 5 ·1992-03-11 ·Pages 1147

Sharrocks AD, Shaw PE

Abstract

暂无摘要

Related Genes

SRF

MeSH Terms

Amino Acid Sequence Base Sequence Molecular Sequence Data Mutagenesis, Site-Directed/genetics Oligodeoxyribonucleotides/genetics Polymerase Chain Reaction

Chemicals

Oligodeoxyribonucleotides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sharrocks A D

Max-Planck-Institut für Immunbiologie, Freiburg, FRG.

Shaw P E

References (4)

4 references, click to expand

Improved site-directed mutagenesis method using PCR.
Nucleic Acids Res. 1991 Aug 25;19(16):4558 PMID: 1886781
Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.
Cell. 1988 Dec 23;55(6):989-1003 PMID: 3203386
Using the polymerase chain reaction to modify expression plasmids for epitope mapping.
Nucleic Acids Res. 1989 Apr 25;17(8):3319 PMID: 2471149
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
Gene. 1990 Nov 30;96(1):125-8 PMID: 2265750

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/20.5.1147

Published

1992-03-11

Pages

1147

Language

English

Region

England

NLM ID

0411011

PMCID

PMC312111

Subset

External Links

PubMed source DOI: 10.1093/nar/20.5.1147 PMC Full Text View journal details

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Improved primer design for PCR-based, site-directed mutagenesis.

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