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PMID: 1549491 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pre-boiling high GC content, mixed primers with 3' complementation allows the successful PCR amplification of Pseudomonas aeruginosa DNA.

Nucleic acids research ·Vol. 20 ·No. 5 ·1992-03-11 ·Pages 1155

Kureishi A, Bryan LE

Abstract

暂无摘要

Related Genes
MeSH Terms
Base Composition/genetics Base Sequence DNA, Bacterial/genetics Molecular Sequence Data Oligodeoxyribonucleotides/genetics Polymerase Chain Reaction/methods Pseudomonas aeruginosa/genetics Temperature
Chemicals
DNA, Bacterial Oligodeoxyribonucleotides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kureishi A
Department of Microbiology & Infectious Diseases, University of Calgary, Alberta, Canada.
Bryan L E
References (3)
3 references, click to expand
  1. DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance.
    J Bacteriol. 1990 Jun;172(6):3481-4 PMID: 2160946
  2. Base pairing involving deoxyinosine: implications for probe design.
    Nucleic Acids Res. 1985 Dec 20;13(24):8927-38 PMID: 4080553
  3. Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.
    J Mol Biol. 1987 Oct 20;197(4):729-36 PMID: 2828631
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1992-03-11
Pages
1155
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312118
Subset
IM
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