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PMID: 2160946 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance.

Journal of bacteriology ·Vol. 172 ·No. 6 ·1990-06-00 ·Pages 3481-4

Hopewell R, Oram M, Briesewitz R, Fisher LM

Abstract

Staphylococcus aureus gyrA and gyrB genes, which encode the DNA gyrase A and B proteins, have been isolated and found to map contiguously. DNA sequence analysis revealed close homology between the S. aureus gyrase subunits and their counterparts in Bacillus subtilis and Escherichia coli, including several conserved amino acid residues whose substitution in E. coli confers resistance to 4-quinolones. These results are discussed in regard to quinolone resistance mechanisms in S. aureus.

MeSH Terms
Amino Acid Sequence Cloning, Molecular DNA Topoisomerases, Type II/genetics DNA, Bacterial/analysis Drug Resistance, Microbial/genetics Genes, Bacterial Molecular Sequence Data Quinolones/pharmacology Staphylococcus aureus/drug effects,genetics
Chemicals
DNA, Bacterial Quinolones DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hopewell R
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, University of London, United Kingdom.
Oram M
Briesewitz R
Fisher L M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.6.3481-3484.1990
Published
1990-06-00
Pages
3481-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209162
Subset
IM
Databases
GENBANK
M37915
External Links
PubMed source DOI: 10.1128/jb.172.6.3481-3484.1990 PMC Full Text View journal details
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