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PMID: 3029692 Published · ppublish English Journal Article

DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.

Nucleic acids research ·Vol. 15 ·No. 2 ·1987-01-26 ·Pages 771-84

Adachi T, Mizuuchi M, Robinson EA, Appella E, O'Dea MH, Gellert M, Mizuuchi K

Abstract

We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Plasmids
Chemicals
Bacterial Proteins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Adachi T
Mizuuchi M
Robinson E A
Appella E
O'Dea M H
Gellert M
Mizuuchi K
References (32)
32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.2.771
Published
1987-01-26
Pages
771-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340466
Subset
IM
Databases
GENBANK
X00870, X04341
External Links
PubMed source DOI: 10.1093/nar/15.2.771 PMC Full Text View journal details
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